This repo holds information on the primers that we have in stock at CGI. Most our primers that we use are described in the literature.
For species where public methods are not stable or are not working in our hands we tend to design new primers based on voucher material from NRM.
We have categorized the primers into four main types:
- Standard primers (for amplification/sanger sequencing)
- NGS primers for metabarcoding
- SSR primers where at least one primers have a flourescent modifications
- qPCR primers which in most cases has a probe sequence that works in conjunction with the primers. See table 2
In addition we also use seperate primers that are only to be used in the forensic lab and hence can not be opened nor moved outside that lab. These are sometimes the same as primers below, but information about all of the forensic primers can be found in section Primers table 3.
Note that the list will over time have sequence and references, but if you are in need of a sequence or reference right now, create a “Issue” on this repo and will update with this information asap.
| Name | Box | Number | Species | Type | Loci | Modification | Reference | Sequence | |
|---|---|---|---|---|---|---|---|---|---|
| LLCBL | 1 | 1 | Lilium lancifolium | Std | CBL | ||||
| LLCBR | 1 | 2 | Lilium lancifolium | Std | CBR | ||||
| Bcal16S_FWD | 1 | 3 | Bacteria | 16S | |||||
| Bcal16S_REV | 1 | 4 | Bacteria | 16S | |||||
| Bcalcytb_FWD | 1 | 5 | Cytb | ||||||
| Bcalcytb_REV | 1 | 6 | Cytb | ||||||
| EluCOI_R | 1 | 8 | Std | Co1 | |||||
| Dbugensis_cytb_F | 1 | 9 | Dreissena bugensis | Std | Cytb | kjsmaö | |||
| Dbugensis_cytb_R | 1 | 10 | Dreissena bugensis | Std | Cytb | ||||
| Dpolymorpha_cytb_F | 1 | 11 | Dreissena polymorpha | Std | Cytb | ||||
| Dpolymorpha_cytb_R | 1 | 12 | Dreissena polymorpha | Cytb | |||||
| SilGla_Dloop_R | 1 | 13 | Silurus glanis | D-loop | |||||
| SilGla_Dloop_L | 1 | 14 | Silurus glanis | D-loop | |||||
| PacifasticusCOI_F | 1 | 15 | Pacifastacus leniusculus | Co1 | |||||
| PacifasticusCOI_R | 1 | 16 | Pacifastacus leniusculus | Co1 | |||||
| AstacusCOIF | 1 | 17 | Astacus astacus | Co1 | |||||
| AstacusCO1R | 1 | 18 | Astacus astacus | Co1 | |||||
| MarMa_16S1.1_F | 1 | 19 | Margaritifera margaritifera | 16S | |||||
| MarMa_16S2.1_F | 1 | 20 | Margaritifera margaritifera | 16S | |||||
| MarMa_16S1.2_R | 1 | 21 | Margaritifera margaritifera | 16S | |||||
| Angcytb_FWD | 1 | 22 | Eel | Cytb | https://www.slu.se/globalassets/ew/org/inst/aqua/externwebb/databaser/kraftdatabasen/delrapport_cgi_slu_edna_2015.pdf | CCTACATGCAAATGGGGCCT | |||
| Angcytb_REV | 1 | 23 | Eel | Cytb | https://www.slu.se/globalassets/ew/org/inst/aqua/externwebb/databaser/kraftdatabasen/delrapport_cgi_slu_edna_2015.pdf | CTCGGGCAATGTGGAGGTAT | |||
| Thymallus_COI_R | 1 | 24 | Thymallus thymallus | Co1 | |||||
| Thymallus_COI_F | 1 | 25 | Thymallus thymallus | Co1 | |||||
| Rut_rutS665 | 1 | 26 | Rutilus rutilus | ||||||
| Rut_rutA665 | 1 | 27 | Rutilus rutilus | ||||||
| T01Mussel16S_F | 1 | 28 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T04Mussel16S_F | 1 | 29 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T06Mussel16S_F | 1 | 30 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T08Mussel16S_F | 1 | 31 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T09Mussel16S_F | 1 | 32 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T13Mussel16S_F | 2 | 33 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T16Mussel16S_F | 2 | 34 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T18Mussel16S_F | 2 | 35 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T01Mussel16S_R | 2 | 36 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T03Mussel16S_R | 2 | 37 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T04Mussel16S_R | 2 | 38 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T05Mussel16S_R | 2 | 39 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T07Mussel16S_R | 2 | 40 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T08Mussel16S_R | 2 | 41 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T09Mussel16S_R | 2 | 42 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T11Mussel16S_R | 2 | 43 | Mussels | NGS | 16sRNA | Adapters for Illumina | |||
| T01_12S_F | 2 | 44 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T04_12S_F | 2 | 45 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T016_12S_F | 2 | 46 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T08_12S_F | 2 | 47 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T09_12S_F | 2 | 48 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T13_12S_F | 2 | 49 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T16_12S_F | 2 | 50 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T18_12S_F | 2 | 51 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T01_12S_R | 2 | 52 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T03_12S_R | 2 | 53 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T04_12S_R | 2 | 54 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T05_12S_R | 2 | 55 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T07_12S_R | 2 | 56 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T08_12S_R | 2 | 57 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T09_12S_R | 2 | 58 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| T11_12S_R | 2 | 59 | Fish | NGS | 12sRNA | Adapters for Illumina | |||
| P2_Bird_sex | 2 | 60 | Birds | Std | |||||
| P3_Bird_sex | 2 | 61 | Birds | Std | |||||
| FR1d_t1 | 2 | 62 | Std | ||||||
| VF2_t1 | 2 | 63 | Std | ||||||
| mlCOIintF | 2 | 64 | Std | Co1 | |||||
| Pe2_F | 3 | 65 | |||||||
| Pe4_F | 3 | 66 | |||||||
| Pe7_F | 3 | 67 | |||||||
| Pe8_F | 3 | 68 | |||||||
| Pe5_F | 3 | 69 | |||||||
| Pe16_F | 3 | 70 | |||||||
| Pe6_F | 3 | 71 | |||||||
| Pe9_R | 3 | 72 | |||||||
| Pe2_R | 3 | 73 | |||||||
| Pe4_R | 3 | 74 | |||||||
| Pe7_R | 3 | 75 | |||||||
| Pe8_R | 3 | 76 | |||||||
| Pe5_R | 3 | 77 | |||||||
| Pe16_R | 3 | 78 | |||||||
| Pe6_R | 3 | 79 | |||||||
| Pe9_F | 3 | 80 | |||||||
| Np641_F | 3 | 81 | |||||||
| Np274_F | 3 | 82 | |||||||
| Np382_F | 3 | 83 | |||||||
| Np207_F | 3 | 84 | |||||||
| Np694_F | 3 | 85 | |||||||
| Uc5_F | 3 | 86 | Unio crassus | SSR | |||||
| Uc15_F | 3 | 87 | Unio crassus | SSR | |||||
| Uc25_F | 3 | 88 | Unio crassus | SSR | |||||
| Np641_R | 3 | 89 | Unio crassus | SSR | |||||
| Np274_R | 3 | 90 | Unio crassus | SSR | |||||
| Np382_R | 3 | 91 | Unio crassus | SSR | |||||
| Np207_R | 3 | 92 | Unio crassus | SSR | |||||
| Np694_R | 3 | 93 | Unio crassus | SSR | |||||
| Uc5_R | 3 | 94 | Unio crassus | SSR | |||||
| Uc15_R | 3 | 95 | Unio crassus | SSR | |||||
| Ucs25_R | 3 | 96 | Unio crassus | SSR | |||||
| Rana temporaria_CO1_F | 4 | 97 | Rana temporaria | Co1 | |||||
| Rana temporaria_CO1_R | 4 | 98 | Rana temporaria | Co1 | |||||
| Rana dalmatina_CO1_R | 4 | 99 | Rana dalmatina | Co1 | |||||
| Rana dalmatina_CO1_F | 4 | 100 | Rana dalmatina | Co1 | |||||
| Rana arvalis_CO1_F | 4 | 101 | Rana arvalis | Co1 | |||||
| Rana arvalis_CO1_R | 4 | 102 | Rana arvalis | Co1 | |||||
| Bufo bufo_CO1_F | 4 | 103 | Bufo bufo | Co1 | |||||
| Bufo bufo_CO1_R | 4 | 104 | Bufo bufo | Co1 | |||||
| Bufotes viridis_CO1_F | 4 | 105 | Bufotes viridis | Co1 | |||||
| Bufotes viridis_CO1_R | 4 | 106 | Bufotes viridis | Co1 | |||||
| DgHCO-2198 | 4 | 107 | Co1 | ||||||
| dgLCO_1490 | 4 | 108 | Co1 | ||||||
| jgHCO2198 | 4 | 109 | Co1 | ||||||
| jgLCO1490 | 4 | 110 | Co1 | ||||||
| LCOech1aF1 | 4 | 111 | Co1 | ||||||
| CBDAsynFor | 4 | 113 | |||||||
| CBDA961Rev | 4 | 114 | |||||||
| THCAsynF | 4 | 115 | |||||||
| THCAsynR | 4 | 116 | |||||||
| C2 | 4 | 117 | |||||||
| E2 | 4 | 118 | |||||||
| TO1reColintF | 4 | 119 | Co1 | Adapters for Illumina | |||||
| T01reHCO2198 | 4 | 120 | Co1 | Adapters for Illumina | |||||
| T03reColintF | 4 | 121 | Co1 | Adapters for Illumina | |||||
| T03reHCO2198 | 4 | 122 | Co1 | Adapters for Illumina | |||||
| T04reCOlintF | 4 | 123 | Co1 | Adapters for Illumina | |||||
| T04reHCO2198 | 4 | 124 | Co1 | Adapters for Illumina | |||||
| T05reCOlintF | 4 | 125 | Co1 | Adapters for Illumina | |||||
| T05reHCO2198 | 4 | 126 | Co1 | Adapters for Illumina | |||||
| T07reCOlintF | 4 | 127 | Co1 | Adapters for Illumina | |||||
| T07reHCO2198 | 4 | 128 | Co1 | Adapters for Illumina | |||||
| T08reCOlintF | 5 | 129 | Co1 | Adapters for Illumina | |||||
| T09reHCO2198 | 5 | 130 | Co1 | Adapters for Illumina | |||||
| T09reCOlintF | 5 | 131 | Co1 | Adapters for Illumina | |||||
| T09reHCO2198 | 5 | 132 | Co1 | Adapters for Illumina | |||||
| T11reCOlintf | 5 | 133 | Co1 | Adapters for Illumina | |||||
| T11reHCO2198 | 5 | 134 | Co1 | Adapters for Illumina | |||||
| T01KarpsF | 5 | 135 | Adapters for Illumina | ||||||
| T02Karp16sR | 5 | 136 | 16s | Adapters for Illumina | |||||
| T02Karp16sF | 5 | 137 | 16s | Adapters for Illumina | |||||
| T04Karp16sR | 5 | 138 | 16s | Adapters for Illumina | |||||
| T04Karp16sF | 5 | 139 | 16s | Adapters for Illumina | |||||
| T07Karp16sR | 5 | 140 | 16s | Adapters for Illumina | |||||
| T05Karp16sF | 5 | 141 | 16s | Adapters for Illumina | |||||
| T08Karp16sR | 5 | 142 | 16s | Adapters for Illumina | |||||
| T08Karp16sF | 5 | 143 | 16s | Adapters for Illumina | |||||
| T10Karp16sR | 5 | 144 | 16s | Adapters for Illumina | |||||
| T10Karp16sF | 5 | 145 | 16s | Adapters for Illumina | |||||
| T11Karp16sR | 5 | 146 | 16s | Adapters for Illumina | |||||
| T12Karp16sF | 5 | 147 | 16s | Adapters for Illumina | |||||
| T12Karp14sR | 5 | 148 | 16s | Adapters for Illumina | |||||
| T13Karp16sF | 5 | 149 | 16s | Adapters for Illumina | |||||
| T14Karp16sR | 5 | 150 | 16s | Adapters for Illumina | |||||
| T01_Mussel16S_F | 5 | 151 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T01Mussel16s1R | 5 | 152 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T02Mussel16s1F | 5 | 153 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T04Mussel16sR | 5 | 154 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T05Mussel16s1F | 5 | 155 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T05Mussel16s1R | 5 | 156 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T10Mussel16s1F | 5 | 157 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T08Mussel16s1R | 5 | 158 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T13Mussel16s1F | 5 | 159 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T12Mussel16s1R | 5 | 160 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T01_16S1 | 6 | 161 | NGS | 16s | Adapters for Illumina | ||||
| T01_16S2 | 6 | 162 | NGS | 16s | Adapters for Illumina | ||||
| T01_F52 | 6 | 163 | NGS | 16s | Adapters for Illumina | ||||
| T01_R193 | 6 | 164 | NGS | 16s | Adapters for Illumina | ||||
| T01_16S3 | 6 | 165 | NGS | 16s | Adapters for Illumina | ||||
| T01_16S4 | 6 | 166 | NGS | 16s | Adapters for Illumina | ||||
| 16SintF | 6 | 167 | 16s | ||||||
| 16SintR | 6 | 168 | 16s | ||||||
| T01_trnL-c | 6 | 169 | NGS | trnL | Adapters for Illumina | ||||
| T07Mussel16s1F | 6 | 170 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T07Mussel16s1R | 6 | 171 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T11Mussel16S1F | 6 | 172 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T11Mussel16S1R | 6 | 173 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T14Mussel16S1F | 6 | 174 | Mussels | NGS | 16s | Adapters for Illumina | |||
| T13Mussel16s1R | 6 | 175 | Mussels | NGS | 16s | Adapters for Illumina | |||
| 494LMod | 6 | 177 | |||||||
| CYTBLmod | 6 | 178 | Cytb | ||||||
| CA1b6_FWD | 6 | 179 | |||||||
| R1CA1b5_FWD | 6 | 180 | |||||||
| CA_1b6_REV | 6 | 181 | |||||||
| R1CA1b5_REV | 6 | 182 | |||||||
| ITS1F_F | 6 | 183 | ITS | ||||||
| ITS4B_R | 6 | 184 | ITS | ||||||
| rbcL_R | 6 | 185 | Rubisco | ||||||
| rbcL_F | 6 | 186 | Rubisco | ||||||
| reHCO2198 | 6 | 187 | |||||||
| reLCO1490 | 6 | 188 | |||||||
| VR1d_t1 | 6 | 189 | |||||||
| VF1d_t1 | 6 | 190 | |||||||
| its1_F | 6 | 191 | ITS | ||||||
| its1_rev | 6 | 192 | ITS | ||||||
| uc69-F | 7 | 193 | Unio crassus | SSR | |||||
| uc69-R | 7 | 194 | Unio crassus | SSR | |||||
| uc77-F | 7 | 195 | Unio crassus | SSR | |||||
| uc77-R | 7 | 196 | Unio crassus | SSR | |||||
| uc7-F | 7 | 197 | Unio crassus | SSR | |||||
| uc7-R | 7 | 198 | Unio crassus | SSR | |||||
| uc16-F | 7 | 199 | Unio crassus | SSR | |||||
| uc16-R | 7 | 200 | Unio crassus | SSR | |||||
| uc39-F | 7 | 201 | Unio crassus | SSR | |||||
| uc39-R | 7 | 202 | Unio crassus | SSR | |||||
| uc19-F | 7 | 203 | Unio crassus | SSR | |||||
| uc19-R | 7 | 204 | Unio crassus | SSR | |||||
| matK_3F | 7 | 205 | MatK | ||||||
| matK_1R | 7 | 206 | MatK | ||||||
| trnLf-trnFr_F | 7 | 207 | trnL | ||||||
| trnLf-trnFr-R | 7 | 208 | trnL | ||||||
| ITS_5 | 7 | 209 | ITS | Plants | |||||
| its4_R | 7 | 210 | ITS | Plants | |||||
| psbAf-trnHr_F | 7 | 211 | trnH | Plants | |||||
| psbAf-trnHr_R | 7 | 212 | trnH | Plants | |||||
| remlCOlintF | 7 | 213 | |||||||
| remlCOlintR | 7 | 214 | |||||||
| DLH-can | 7 | 215 | |||||||
| Thr1 | 7 | 216 | |||||||
| ITS1F | 7 | 217 | ITS | ||||||
| LR3 | 7 | 218 | |||||||
| 5.8S Chytr | 7 | 219 | |||||||
| ITS1-3 Chytr | 7 | 220 | ITS | ||||||
| Phyllo COI_FWD | 7 | 221 | |||||||
| Phyllo_COI_REV | 7 | 222 | |||||||
| igsF | 7 | 223 | |||||||
| igsR | 7 | 224 | |||||||
| LO | 8 | 225 | |||||||
| H1046 | 8 | 226 | |||||||
| dogdl2_R | 8 | 227 | |||||||
| dogdl1g_F | 8 | 228 | |||||||
| Ele-ND5-F3 | 8 | 229 | |||||||
| Ele-ND5-R3 | 8 | 230 | |||||||
| Ele-ND5-F1 | 8 | 231 | |||||||
| Ele-ND5-R1 | 8 | 232 | |||||||
| 16S-Frag1aR | 8 | 233 | TCCAAGGTCGCCCCAACCGAA | ||||||
| 16S-Frag1aF | 8 | 234 | CATAAGACGGAGAAGACCCCTGTGGA | ||||||
| 16S-Frag2aF | 8 | 235 | CCTTGGAGAAAAACAAANCCTCAAA | ||||||
| 16S-Frag2aR | 8 | 236 | TCCCTGGGGTAGCTTGGTCCAT | ||||||
| Mam16S1 | 8 | 237 | |||||||
| Mam16S2 | 8 | 238 | |||||||
| Lx1R | 8 | 239 | |||||||
| Lx1F | 8 | 240 | |||||||
| Fisk2_FWD | 8 | 241 | |||||||
| MiFish_U-R | 8 | 242 | |||||||
| Vert_16S_F1 | 8 | 243 | |||||||
| Vert_16S_R1 | 8 | 244 | |||||||
| CHD_1F | 8 | 245 | |||||||
| CHD_1R | 8 | 246 | |||||||
| Mussels ITS-1F | 8 | 247 | |||||||
| Mussels ITS-1R | 8 | 248 | |||||||
| MiFish_U-F | 8 | 249 | |||||||
| 12S_V5_F | 9 | 250 | |||||||
| 12S_V5_R | 9 | 251 | |||||||
| 16S-Frag1aF_M13_F | 9 | 252 | |||||||
| 16S-Frag1aR_M13_R | 9 | 253 | |||||||
| 16S-Frag2aF_M13_F | 9 | 254 | |||||||
| 16S-Frag2aR_M13_R | 9 | 255 | |||||||
| MiFish-U-F_M13F | 9 | 256 | |||||||
| MiFish-U-R_M13R | 9 | 257 | |||||||
| 12S-V5_F_M13F | 9 | 258 | |||||||
| 12S-V5_R_M13R | 9 | 259 | |||||||
| M13_F | 9 | 260 | |||||||
| M13_R | 9 | 261 | |||||||
| 12S_1380R_M13R | 9 | 262 | Pisces | 12S | |||||
| 12S_30F_M13_F | 9 | 263 | Pisces | 12S | |||||
| L152 | 9 | 264 | Cyprinidae | Cytb | |||||
| H165 | 9 | 265 | Cyprinidae | Cytb | |||||
| BC_F3 | 9 | 266 | Mammals | COI | CCCCTATTCGTATGATCAGTATTAATTAC | ||||
| BC_R2 | 9 | 267 | Mammals | COI | TAAACCTCAGGATGTCCGAAGAATCA | ||||
| ZF_FWD | 9 | 268 | ZF | Sex | AAGTTTACACAACCACCTGG | ||||
| ZF_REV | 9 | 269 | ZF | Sex | CACAGAATTTACACTTGTGCA | ||||
| BF1 | 9 | 270 | ACWGGWTGRACWGTNTAYCC | ||||||
| BF2 | 9 | 271 | GCHCCHGAYATRGCHTTYCC | ||||||
| BR1 | 9 | 272 | ARYATDGTRATDGCHCCDGC | ||||||
| BR2 | 9 | 273 | TCDGGRTGNCCRAARAAYCA | ||||||
| Nitella_meta_FWD | 9 | 274 | CAYCGTGCTATGCATGCTGT | ||||||
| Nitella_meta_REV | 9 | 275 | AGGCATRTGCCAAACATGA | ||||||
| P8 | 9 | 276 | CTCCCAAGGATGAGRAAYTG | ||||||
| LGL331 | 9 | 277 | -CAA-ATC-ATG-CAA-GGA-TAG-AC- | ||||||
| LGL335 | 9 | 278 | -AGA-CCT-GAT-TCC-AGA-CAG-TAC-CA- | ||||||
| cpITS3 | 9 | 279 | TCCTGGCGTCGAGCTATTTTTCC | ||||||
| cpITS2 | 9 | 280 | CCGGATAACTGCTGAAAGCATC | ||||||
| LCO1490 | 9 | 281 | Insects | COI | GGTCAACAAATCATAAAGATATTGG | ||||
| HCO2198 | 9 | 282 | Insects | COI | TAAACTTCAGGGTGACCAAAAAATCA | ||||
| MiDeca_F | 10 | 283 | |||||||
| MiDeca_R | 10 | 284 | |||||||
| Illumina_MiFish_U_R | 10 | 285 | |||||||
| Illumina_MiFish_U_F | 10 | 286 | |||||||
| Gulaerla_R | 10 | 287 | GGTGGGTGAGTTGGGTAATG | ||||||
| Gulaerla_F | 10 | 288 | CWATCTCGAGCAACCACTGA | ||||||
| FR1d | 10 | 289 | Fish | Standard | COI | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | ACCTCAGGGTGTCCGAARAAYCARAA | ||
| FishR2 | 10 | 290 | Fish | Standard | COI | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | ACTTCAGGGTGACCGAAGAATCAGAA | ||
| FishF2 | 10 | 291 | Fish | Standard | COI | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | CGACTAATCATAAAGATATCGGCAC | ||
| VF2 | 10 | 292 | Fish | Standard | COI | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | CAACCAACCACAAAGACATTGGCAC | ||
| FishR2_t1 | 10 | 293 | Fish | Standard | COI | with M13 | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | CAGGAAACAGCTATGACACTTCAGGGTGACCGAAGAATCAGAA | |
| Fishf2_t1 | 10 | 294 | Fish | Standard | COI | with M13 | Ivanova et al.2007, Universal primer cocktail for fish DNA barcoding | TGTAAAACGACGGCCAGTCGACTAATCATAAAGATATCGGCAC | |
| SaxND2R4 | 10 | 295 | Bird | Standard | mtND2 | J.M. Collinson, 2012. A genetic analysis of the first british siberian stonechat | GGCAGGAAGCCTGTTAAAGG | ||
| SaxND2F4 | 10 | 296 | Bird | Standard | mtND2 | J.M. Collinson, 2012. A genetic analysis of the first british siberian stonechat | GCTGAATGGCTATCATTATCG | ||
| SaxND2R2 | 10 | 297 | Bird | Standard | mtND2 | J.M. Collinson, 2012. A genetic analysis of the first british siberian stonechat | CCGGTYTGTCATGCGTTAG | ||
| SaxND2F1 | 10 | 298 | Bird | Standard | mtND2 | J.M. Collinson, 2012. A genetic analysis of the first british siberian stonechat | ACCTCCAGCCTACTCCTAG | ||
| L15774_M13F | 10 | 299 | Martes | Standard | cytb | wit hM13 | Davison A, Birks JD, Brookes RC, Messenger JE, Griffiths HI. Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Mol Ecol. 2001 Oct;10(10):2479-88. doi: 10.1046/j.1365-294x.2001.01381.x. PMID: 11742548. | ACATGAATTGGAGGACAACCAGT | |
| H16498_M13 | 10 | 300 | Martes | Standard | cytb | witn M13 | Davison A, Birks JD, Brookes RC, Messenger JE, Griffiths HI. Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Mol Ecol. 2001 Oct;10(10):2479-88. doi: 10.1046/j.1365-294x.2001.01381.x. PMID: 11742548. | CCTGAACTAGGAACCAGATG | |
| LRCB1_M13F | 10 | 301 | Martes | Standard | cytb | with M13 | Davison A, Birks JD, Brookes RC, Messenger JE, Griffiths HI. Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Mol Ecol. 2001 Oct;10(10):2479-88. doi: 10.1046/j.1365-294x.2001.01381.x. PMID: 11742548. | TGGTCTTGTAAACCAAAAATGG | |
| LRCB3_M13 | 10 | 302 | Martes | Standard | cytb | with M13 | Davison A, Birks JD, Brookes RC, Messenger JE, Griffiths HI. Mitochondrial phylogeography and population history of pine martens Martes martes compared with polecats Mustela putorius. Mol Ecol. 2001 Oct;10(10):2479-88. doi: 10.1046/j.1365-294x.2001.01381.x. PMID: 11742548. | AGACTCAAGGAAGAAGCAAC | |
| 341F_Illumina | 10 | 303 | Illumina | ACACTCTTTCCCTACACGACGCTCTTCCGATCTCCTACGGGNGGCWGCAG | |||||
| 805R_Illumina | 10 | 304 | Illumina | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTGACTACHVGGGTATCTAATCC | |||||
| Anisakidae16sF | 10 | 305 | nematodes | standart | GCGTGAGGACATTAAGGTAGC | ||||
| Anisakidae16sR | 10 | 306 | nematodes | standart | |||||
| P. lessonae cytC1_676_F | 10 | no number | gölgroda | standart | |||||
| P. lessonae cytC1_850_R | 10 | no number | gölgroda | standart | |||||
| CGAAGACWTATCTTTGTTTA | |||||||||
| G10L_R | 11 | 307 | bears | standard | ssr | GATACAGAAACCTACCCATGCG | |||
| G10L_F | 11 | 308 | bears | standard | ssr | CAGGACAGGATATTGACATTGA | |||
| MU51_F | 11 | 309 | bears | standard | ssr | GCCAGAATCCTAAGAGACCT | |||
| MU51_R | 11 | 310 | bears | standard | ssr | GTTTCTTGAAAGGTTAGATGGAAGAGATG | |||
| MU59_F | 11 | 311 | bears | standard | ssr | GCTGCTTTGGGACATTGTAA | |||
| MU59_R | 11 | 312 | bears | standard | ssr | GTTTCTTCAATCAGGCATGGGGAAGAA | |||
| MU50_F | 11 | 313 | bears | standard | ssr | GTCTCTGTCATTTCCCCATC | |||
| MU50_R | 11 | 314 | bears | standard | ssr | GAGCAGGAAACATGTAAGATG | |||
| MU05_F | 11 | 315 | bears | standard | ssr | ATGTGGATACAGTGGAATAGACC | |||
| MU05_R | 11 | 316 | bears | standard | ssr | GTTTCTTGTGACATGAACTGAAACTTGTTAT | |||
| MU09_F | 11 | 317 | bears | standard | ssr | GCCAGCATGTGGGTATATGTGT | |||
| MU09_R | 11 | 318 | bears | standard | ssr | GTTTCTTAGCAGCATATTTTTGGCTTTGAAT | |||
| MU10_F | 11 | 319 | bears | standard | ssr | TTCAGATTTCATCAGTTTGAC | |||
| MU10_R | 11 | 320 | bears | standard | ssr | TTTGTATCTTGGTTGTCAGC | |||
| MU23_F | 11 | 321 | bears | standard | ssr | GCCTGTGTGCTATTTTATCC | |||
| MU23_R | 11 | 322 | bears | standard | ssr | GTTTCTTTTGCTTGCCTAGACCACC | |||
| Se47_F | 11 | 323 | bears | standard | ssr | ||||
| 143Rmod | 11 | 324 | bears | standard | ssr | ||||
| Ca1_F | 12 | 325 | Fish | ssr | NED | AAGACGATGCTGGATGTTTAC | |||
| Ca1_R | 12 | 326 | Fish | ssr | CTATAGCTTATCCCGGCAGTA | ||||
| LleC090_F | 12 | 327 | Fish | ssr | VIC | TCAGACACAACTAACCGACC | |||
| LleC090_R | 12 | 328 | Fish | ssr | GGCGCTGTCCAGAACTGA | ||||
| LCO1_F | 12 | 329 | Fish | ssr | NED | CACGGGACAATTTGGATGTTTTAT | |||
| LCO1_R | 12 | 330 | Fish | ssr | AGGGGGCAGCATACAAGAGACAAC | ||||
| LleA029_F | 12 | 331 | Fish | ssr | NED | TTTACCAGCATCCCCCAT | |||
| LleA029_r | 12 | 332 | Fish | ssr | CATTTCACTCACTGAAGGAGAAC | ||||
| LleA150_F | 12 | 333 | Fish | ssr | PET | AAAGTGTAAATCCAGATGTTTAAGT | |||
| LleA150_r | 12 | 334 | Fish | ssr | AAAGGATAATTTTCAGAGTAACGAG | ||||
| BL130_F | 12 | 335 | Fish | ssr | PET | GCGATGGCACTGATGGA | |||
| BL130_R | 12 | 336 | Fish | ssr | TAAAGAGCTCGTTTTGAAGCG | ||||
| LceC1_F | 12 | 337 | Fish | ssr | 6FAM | AGGTGTTGGTTCCTCCCG | |||
| LceC1_R | 12 | 338 | Fish | ssr | TGTTATCTCGGTTTCACGAGC | ||||
| Lsou05_F | 12 | 339 | Fish | ssr | 6FAM | CTGAAGAAGACCCTGGTTCG | |||
| Lsou05_R | 12 | 340 | Fish | ssr | CCCACATCTGCTGACTCTGAC | ||||
| BL1T2_F | 12 | 341 | Fish | ssr | PET | TGTTTGCTCAGACAAAACTTTCA | |||
| BL1T2_R | 12 | 342 | Fish | ssr | ACGGGTCTCAGATGATGCTC | ||||
| CypG24_F | 12 | 343 | Fish | ssr | PET | CTGCCGCATCAGAGATAAACACTT | |||
| CypG24_R | 12 | 344 | Fish | ssr | TGGCGGTAAGGGTAGACCAC | ||||
| N7K4_F | 12 | 345 | Fish | ssr | NED | ACGAGCATCAGTATCCAGAGACAC | |||
| N7K4_R | 12 | 346 | Fish | ssr | CATGTTTCCACATCTGAGCTAAAA | ||||
| BL161_F | 12 | 347 | Fish | ssr | VIC | GACTCGCAGAGCTCCTTCAC | |||
| BL161_R | 12 | 348 | Fish | ssr | GGCGCTTGATTCAGTCTTTC | ||||
| Z21908_F | 12 | 349 | Fish | ssr | PET | ATTGATTAGGTCATTGCCCG | |||
| Z21908_R | 12 | 350 | Fish | ssr | AGGAGTCATCGCTGGTGAGT | ||||
| BL1_2b_F | 12 | 351 | Fish | ssr | 6FAM | TTTGCACTAGTAACGAGCATCA | |||
| BL1_2b_R | 12 | 352 | Fish | ssr | CAGCACAGTTTCTCCATCCA | ||||
| BL1_153_F | 12 | 353 | Fish | ssr | 6FAM | GCACAGCTCTAATCGGTCACT | |||
| BL1_153_R | 12 | 354 | Fish | ssr | TATGGTCAAACACGGGTCAA | ||||
| Lsou34_F | 12 | 355 | Fish | ssr | VIC | CCAGACAGGGTGATGATTCC | |||
| Lsou34_r | 12 | 356 | Fish | ssr | GTAGCGACGTTCAGGTCTCG | ||||
| Rser10_F | 12 | 357 | Fish | ssr | 6FAM | TGCGTAATCGTGAAGCGGTG | |||
| Rser10_R | 12 | 358 | Fish | ssr | GCCACTAAAGCGCAGAAGCC | ||||
| Lid8_F | 12 | 359 | Fish | ssr | VIC | AAATGCTAATGTTTCATCCATA | |||
| Lid8_R | 12 | 360 | Fish | ssr | AAGCCTTCCTCTTGTTCC | ||||
| BL1_98_F | 12 | 361 | Fish | ssr | PET | ATTGTTTTCATTTTGTCAG | |||
| BL1_98_R | 12 | 362 | Fish | ssr | CCGAGTGTCAGAGTTATT | ||||
| CtoF_172_F | 12 | 363 | Fish | ssr | VIC | ACCAAGGTGAAAGCCTGTAA | |||
| CtoF_172_R | 12 | 364 | Fish | ssr | GGACACGATGACAACGG | ||||
| Hg1_4 _F | 13 | 365 | Seals | ssr | 6FAM | CTCCAAGACGACTGAAACCC | |||
| Hg1_4 _R | 13 | 366 | Seals | ssr | TACCATATCTTTGTGGCTCTG | ||||
| Hg2_3 _F | 13 | 367 | Seals | ssr | NED | CCAATGACAACTTACTGAGAAT | |||
| Hg2_3 _R | 13 | 368 | Seals | ssr | TGTGAAGTGCTCTGTTTTGC | ||||
| Hg3_6 _F | 13 | 369 | Seals | ssr | PET | AGATCACATTCTTTTTATGGCTG | |||
| Hg3_6 _R | 13 | 370 | Seals | ssr | GATTGGATAAAGAAGATGTGAGGG | ||||
| Hg4_2 _F | 13 | 371 | Seals | ssr | NED | AATCGAAATGCTGAGCCTCC | |||
| Hg4_2 _R | 13 | 372 | Seals | ssr | TGATTTGACTTCCCTTCCCTG | ||||
| Hg6_1_F | 13 | 373 | Seals | ssr | PET | TGCACCAGAGCCTAAGCAGACTG | |||
| Hg6_1_R | 13 | 374 | Seals | ssr | CCACCAGCCAGTTCACCCAG | ||||
| Hg8_9_F | 13 | 375 | Seals | ssr | 6FAM | TGTTAACTATCTGGCACAGAGTAAG | |||
| Hg8_9_R | 13 | 376 | Seals | ssr | TTTCCTATGGGTTCTACTCTCAG | ||||
| Hg8_10_F | 13 | 377 | Seals | ssr | VIC | AATTCTGAAGCAGCCCAAG | |||
| Hg8_10_R | 13 | 378 | Seals | ssr | GAATTCTTTTCTAGCATAGGTTG | ||||
| Hgdii_F | 13 | 379 | Seals | ssr | NED | ACCTGCCATAGTGCTCATC | |||
| Hgdii_R | 13 | 380 | Seals | ssr | AGGACTCCTGCCACTGAGAA | ||||
| Hl15_F | 13 | 381 | Seals | ssr | NED | CATCTTGTAGTGCCAAAAAC | |||
| Hl15_R | 13 | 382 | Seals | ssr | ATCTTTCAGTTGACCCTTCT | ||||
| Pvc19_F | 13 | 383 | Seals | ssr | NED | GGGTGAACAGGATTTATCC | |||
| Pvc19_R | 13 | 384 | Seals | ssr | GTGCTAGATAACAATCCTAC | ||||
| Pvc26_F | 13 | 385 | Seals | ssr | NED | TTTTCTCCATACCTACATAAT | |||
| Pvc26_R | 13 | 386 | Seals | ssr | ATTGTGATCCCATTTTTGTAA | ||||
| Pvc30_F | 13 | 387 | Seals | ssr | 6FAM | GCATGTGATCTTACAGCAAT | |||
| Pvc30_R | 13 | 388 | Seals | ssr | CATGGGTTCTCAATAGAAGA | ||||
| Pvc78_F | 13 | 389 | Seals | ssr | VIC | GAGTATACCTCCATACTACAC | |||
| Pvc78_R | 13 | 390 | Seals | ssr | AGTTGTTCTCCTGACCCAAG | ||||
| SGPv9_F | 13 | 391 | Seals | ssr | VIC | TAGTGTTTGGAAATGAGTTGGC | |||
| SGPv9_R | 13 | 392 | Seals | ssr | CTGATCCTTGTGAATCCCAGC | ||||
| SGPv10_F | 13 | 393 | Seals | ssr | VIC | TTCACTTAGCATAATTCCCTC | |||
| SGPv10_R | 13 | 394 | Seals | ssr | TCATGAATTGGTATTAGACAAAG | ||||
| SGPv11_F | 13 | 395 | Seals | ssr | 6FAM | CAGAGTAAGCACCCAAGGAGCAG | |||
| SGPv11_R | 13 | 396 | Seals | ssr | GTGCTGGTGAATTAGCCCATTATAAG | ||||
| SGPv16_F | 13 | 397 | Seals | ssr | 6FAM | AGCTAGTGTTAATGATGGTGTG | |||
| SGPv16_R | 13 | 398 | Seals | ssr | TCTGAGAGATTCAGAGTAACCTTC | ||||
| Pacifastacus leniusculus_FWD | 14 | 399 | Crayfish | TGTAGTCACGGCACATGCTT | |||||
| Pacifastacus leniusculus_REV | 14 | 400 | Crayfish | CCAGTACCCACTCCTC | |||||
| Astacus astacus_FWD | 14 | 401 | Crayfish | TCGGTCAACCTGGGAGATTA | |||||
| Astacus astacus_REV | 14 | 402 | Crayfish | GAAAAGGGGAGCAATCAAAA | |||||
| Aphanomyces astaci_FWD | 14 | 403 | Crayfish plaque | TGTCTTTGGACGAAGCAGAA | |||||
| Aphanomyces astaci_REV | 14 | 404 | Crayfish plaque | GCCGAAACAAGCAAAACT | |||||
| Oenanthe_ND1_FWD | 14 | 405 | Bird | CATGAACATCCTCCCCAATC | |||||
| Oenanthe_ND1_REV | 14 | 406 | Bird | GGTTTGGGCTACTGCCGTA | |||||
| FishU_R | 14 | 407 | Fish | metabarcoding | CATGATGCAAAAGGTACRRG | ||||
| FishU_F | 14 | 408 | Fish | metabarcoding | ACAYACCGCCCGTCACYCTC | ||||
| 12S1 | 14 | 409 | Fish | metabarcoding | GACAGCTACGACACAAACTGCGATTAGATACC | ||||
| 12S2 | 14 | 410 | Fish | metabarcoding | TGCACCTTCCAGTACACTTACCATGTTACGAC | ||||
| teleoR | 14 | 411 | Fish | metabarcoding | CTTCCGGTACACTTACCATG | ||||
| V05F_898 | 14 | 412 | Fish | metabarcoding | AAACTCGTGCCAGCCACC | ||||
| M13_F | 14 | 413 | E. Coli | LacZ | https://www.thermofisher.com/order/catalog/product/N52002 | - | |||
| M13_R | 14 | 414 | E. Coli | LacZ | https://www.thermofisher.com/order/catalog/product/N53002 | - | |||
| Potamogeton_seq_REV | 14 | 415 | Aquatic plants | - | - | ||||
| Potamogeton_seq_FWD | 14 | 416 | Aquatic plants | - | - | ||||
| atpB-2F | 14 | 417 | Plants | atpB | - | ||||
| rbcL-2R | 14 | 418 | Plants | rbcL | - | ||||
| Lamprothamnium771_FWD | 14 | 419 | Algae | - | - | ||||
| Lamprothamnium1056_REV | 14 | 420 | Algae | - | - | ||||
| Zostera_nolti_92_FWD | 14 | 421 | Seagrass | - | - | ||||
| Zostera_nolti_247_REV | 14 | 422 | Seagrass | - | - | ||||
| Lamprothamnium697_FWD | 14 | 423 | Algae | - | - | ||||
| Lamprothamnium1130_REV | 14 | 424 | Algae | - | - | ||||
| Zostera_nolti_82_FWD | 14 | 425 | Seagrass | - | - | ||||
| Zostera_nolti_352_REV | 14 | 426 | Seagrass | - | - | ||||
| Ceph18S_fwd | 14 | 427 | Cephalopod | 18sRNA | de Jonge, Danielle & Merten, Véronique & Bayer, Till & Puebla, Oscar & Reusch, Thorsten & Hoving, H.. (2021). A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region. Royal Society Open Science. 8. 201388. 10.1098/rsos.201388. | CGCGGCGCTACATATTAGAC | |||
| Ceph18S_rev | 14 | 428 | Cephalopod | 18sRNA | de Jonge, Danielle & Merten, Véronique & Bayer, Till & Puebla, Oscar & Reusch, Thorsten & Hoving, H.. (2021). A novel metabarcoding primer pair for environmental DNA analysis of Cephalopoda (Mollusca) targeting the nuclear 18S rRNA region. Royal Society Open Science. 8. 201388. 10.1098/rsos.201388. | GCACTTAACCGACCGTCGAC | |||
| CephMLSf1 | 14 | 429 | Cephalopod | mt16sRNA | Jarman, Simon & Redd, Kevin & GALES, N.. (2005). Group-specific primers for amplifying DNA sequences that identify Amphipoda, Cephalopoda, Echinodermata, Gastropoda, Isopoda, Ostracoda and Thoracica. Molecular Ecology Notes. 6. 268 - 271. 10.1111/j.1471-8286.2005.01172.x. | TGC GGTATTWTAACTGTACT | |||
| CephMLSr1 | 14 | 430 | Cephalopod | mt16sRNA | Jarman, Simon & Redd, Kevin & GALES, N.. (2005). Group-specific primers for amplifying DNA sequences that identify Amphipoda, Cephalopoda, Echinodermata, Gastropoda, Isopoda, Ostracoda and Thoracica. Molecular Ecology Notes. 6. 268 - 271. 10.1111/j.1471-8286.2005.01172.x. | TTATTCCTTRATCACCC | |||
| Lanius_1804F | 14 | 431 | Lanius | - | ACGCTATTYTACGATCAATCCCCA | ||||
| Lanius_1903R | 14 | 432 | Lanius | - | TTGGARTTGTGGAGTAGYGGGAT | ||||
| Illumina_gITS7ngs_fwd | 14 | 433 | - | - | ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTGARTCATCRARTYTTTG | ||||
| Illumina_ITS4ngsUni_rev | 14 | 434 | - | - | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCTSCSCTTANTDATATGC | ||||
| tmT a (UGU) | 15 | 435 | - | - | - | ||||
| tmL b (UAA) | 15 | 436 | - | - | - | ||||
| tmL d (UAA) | 15 | 437 | - | - | - | ||||
| batra_F_L3541 | 15 | 438 | - | - | - | ||||
| batra_R_H3596 | 15 | 439 | - | - | - | ||||
| 2550F | 15 | 440 | - | - | - | ||||
| 2718R | 15 | 441 | - | - | - | ||||
| Illumina_18S-EUK581-F | 15 | 442 | - | - | ACACTCTTTCCCTACACGACGCTCTTCCGATCT GTGCCAGCAGCCGCG | ||||
| Illumina_18S-EUK1134-R | 15 | 443 | - | - | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCT TTTAAGTTTCAGCCTTGCG | ||||
| Anthrenochernes_stellae_COI_F | 15 | 444 | - | - | - | ||||
| Anthrenochernes_stellae_COI_R | 15 | 445 | - | - | - | ||||
| Osmoderma_eremita_COI_FWD | 15 | 446 | - | - | - | ||||
| Osmoderma_eremita_COI_REV | 15 | 447 | - | - | - | ||||
| rs24198287R | 15 | 448 | - | - | GGCATGATTCCTGGTGGTTTTC | ||||
| rs24198287F | 15 | 449 | - | - | GTAGGCCAGTCTTCAGTGTGGTGA | ||||
| rs22835438F | 15 | 450 | - | - | GGGATTGAACCCCTGACCTGAGT | ||||
| rs22835438R | 15 | 451 | - | - | TGAACCATGACCACCCCTCGT | ||||
| rs23608542F | 15 | 452 | - | - | ACTTCTCCACGCGGAAACACTT | ||||
| rs23608542R | 15 | 453 | - | - | GAAATGGAAGGTGGCATTAGAACC | ||||
| rs24163825F | 15 | 454 | - | - | GCTAGTCCTATGCCCTGAAACTCAT | ||||
| rs24163825R | 15 | 455 | - | - | TCCTTATGAGCCTTAGTTTTGCTACCT | ||||
| rs24189603F | 15 | 456 | - | - | GGAGCTCGGAGTGACTATACTGGAAT | ||||
| rs24189603R | 15 | 457 | - | - | CCTCGGATGCAGTCTGTTTCTCTG | ||||
| Illumina_16S 926_rev | 15 | 458 | - | - | GTGACTGGAGTTCAGACGTGTGCTCTTCCGATCTCCGYCAATTYMTTTRAGTTT | ||||
| Illumina_16S_515_fwd | 15 | 459 | - | - | ACACTCTTTCCCTACACGACGCTCTTCCGATCTGTGYCAGCMGCCGCGGTAA | ||||
| BF3 | 15 | 460 | - | - | - | ||||
| BR3 | 15 | 461 | - | - | - | ||||
| Unionioda_FWD | 15 | 462 | - | - | - | ||||
| Unionioda_REV | 15 | 463 | - | - | - | ||||
| Mcb_KPF398 | 15 | 464 | - | - | - | ||||
| Mcb_KPR869 | 15 | 465 | - | - | - | ||||
| Cyp_1_FWD | 15 | 466 | - | - | - | ||||
| Cyp_2_FWD | 15 | 467 | - | - | - | ||||
| MarVer3F | 15 | 468 | - | - | - | ||||
| MarVer3R | 15 | 469 | - | - | - | ||||
| LepF2_t1 | 15 | 470 | - | - | - | ||||
| Tc4-F | 16 | 471 | Tilia cordata (tree) | - | - | ||||
| Tc4-R | 16 | 472 | Tilia cordata (tree) | - | - | ||||
| Tc5-F | 16 | 473 | Tilia cordata (tree) | - | - | ||||
| Tc5-R | 16 | 474 | Tilia cordata (tree) | - | - | ||||
| Tc6-F | 16 | 475 | Tilia cordata (tree) | - | - | ||||
| Tc6-R | 16 | 476 | Tilia cordata (tree) | - | - | ||||
| Tc7-F | 16 | 477 | Tilia cordata (tree) | - | - | ||||
| Tc7-R | 16 | 478 | Tilia cordata (tree) | - | - | ||||
| Tc8-F | 16 | 479 | Tilia cordata (tree) | - | - | ||||
| Tc8-R | 16 | 480 | Tilia cordata (tree) | - | - | ||||
| Tc915-F | 16 | 481 | Tilia cordata (tree) | - | - | ||||
| Tc915-R | 16 | 482 | Tilia cordata (tree) | - | - | ||||
| Tc927-F | 16 | 483 | Tilia cordata (tree) | - | - | ||||
| Tc927-R | 16 | 484 | Tilia cordata (tree) | - | - | ||||
| Tc937-F | 16 | 485 | Tilia cordata (tree) | - | - | ||||
| Tc937-R | 16 | 486 | Tilia cordata (tree) | - | - | ||||
| Tc943-F | 16 | 487 | Tilia cordata (tree) | - | - | ||||
| Tc943-R | 16 | 488 | Tilia cordata (tree) | - | - | ||||
| Tc951-F | 16 | 489 | Tilia cordata (tree) | - | - | ||||
| Tc951-R | 16 | 490 | Tilia cordata (tree) | - | - | ||||
| Tc963-F | 16 | 491 | Tilia cordata (tree) | - | - | ||||
| Tc963-R | 16 | 492 | Tilia cordata (tree) | - | - | ||||
| Mm2209-F | 16 | 493 | - | - | - | ||||
| Mm2209-R | 16 | 494 | - | - | - | ||||
| Mm2230-F | 16 | 495 | - | - | - | ||||
| Mm2230-R | 16 | 496 | - | - | - | ||||
| Mm2233-F | 16 | 497 | - | - | - | ||||
| Mm2233-R | 16 | 498 | - | - | - | ||||
| Mm2235-F | 16 | 499 | - | - | - | ||||
| Mm2235-R | 16 | 500 | - | - | - | ||||
| Mm2236-F | 16 | 501 | - | - | - | ||||
| Mm2236-R | 16 | 502 | - | - | - | ||||
| Mm2238-F | 16 | 503 | - | - | - | ||||
| Mm2238-R | 16 | 504 | - | - | - | ||||
| MarMa3116-F | 16 | 505 | - | - | - | ||||
| MarMa3116-R | 16 | 506 | - | - | - | ||||
| MarMa3621-F | 17 | 507 | - | - | - | ||||
| MarMa3621-R | 17 | 508 | - | - | - | ||||
| MarMa4277-F | 17 | 509 | - | - | - | ||||
| MarMa4277-R | 17 | 510 | - | - | - | ||||
| MarMa4315-F | 17 | 511 | - | - | - | ||||
| MarMa4315-R | 17 | 512 | - | - | - | ||||
| MarMa5167-F | 17 | 513 | - | - | - | ||||
| MarMa5167-R | 17 | 514 | - | - | - | ||||
| SGPv11F | 17 | 515 | - | - | - | ||||
| Hg1_4F | 17 | 516 | - | - | - | ||||
| 18S-EUK581-F | 17 | 517 | Eukaryotes | - | - | ||||
| 18S-EUK1134-R | 17 | 518 | Eukaryotes | - | - | ||||
| Moose CR 1F | 17 | 519 | Alces alces | - | - | ||||
| Moose CR 1R | 17 | 520 | Alces alces | - | - | ||||
| Moose CR 2F | 17 | 521 | Alces alces | - | - | ||||
| Moose CR 2R | 17 | 522 | Alces alces | - | - | ||||
| Moose CR 3F | 17 | 523 | Alces alces | - | - | ||||
| Moose CR 3R | 17 | 524 | Alces alces | - | - | ||||
| Aacytb1044_FWD | 17 | 525 | Alces alces | - | - | ||||
| Aacytb1044_REV | 17 | 526 | Alces alces | - | - | ||||
| Cccytb150_FWD | 17 | 527 | Capreolus capreolus | - | - | ||||
| Cccytb150_REV | 17 | 528 | Capreolus capreolus | - | - | ||||
| Cdcytb230_FWD | 17 | 529 | Cervus dama | - | - | ||||
| Cdcytb230_REV | 17 | 530 | Cervus dama | - | - | ||||
| Cecytb412_FWD | 17 | 531 | Cervus elaphus | - | - | ||||
| Cecytb412_REV | 17 | 532 | Cervus elaphus | - | - | ||||
| 12SCC_FW | 17 | 533 | - | - | |||||
| 12SCE_FW | 17 | 534 | - | - | |||||
| 12SDD_FW | 17 | 535 | - | - | |||||
| 12SCERV_REV | 17 | 536 | - | - | |||||
| PRT_F | 17 | 537 | - | - | |||||
| PRT_R | 17 | 538 | - | - | |||||
| A2_Mluc_FW | 17 | 539 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| A2_Mluc_REV | 17 | 540 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| G6_Mluc_FW | 17 | 541 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| G6_Mluc_REV | 17 | 542 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| EF15_Mluc_FW | 18 | 543 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| EF15_Mluc_REV | 18 | 544 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| clone_A2_Mluc_FW | 18 | 545 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| clone_A2_Mluc_REV | 18 | 546 | Bats | Standard | - | Jan C et al. Development of conserved microsatellite markers of high cross-species utility in bat species (Vespertilionidae, Chiroptera, Mammalia). Mol Ecol Resour. 2012 May;12(3):532-48. doi: 10.1111/j.1755-0998.2012.03114.x | - | ||
| ITS-mod_FWD | 18 | 547 | - | - | |||||
| 26S25_Rev | 18 | 548 | - | - | |||||
| illuminaKarpR | 18 | 549 | Karp | - | - | ||||
| illuminaKarpF | 18 | 550 | Karp | - | - | ||||
| illumina-ITSmusselsF | 18 | 551 | Mussels | - | - | ||||
| illumina-ITSmusselsR | 18 | 552 | Mussels | - | - | ||||
| illumina-UnionidaR | 18 | 553 | Unionida | - | - | ||||
| illumina-UnionidaF | 18 | 554 | Unionida | - | - | ||||
| illumina-12S-V5-F | 18 | 555 | Mammals | - | - | ||||
| illumina-12S-V5_R | 18 | 556 | Mammals | - | - | ||||
| illumina-V16S-U_REV | 18 | 557 | Vertebrates | - | - | ||||
| illumina-V16S-U_FWD | 18 | 558 | Vertebrates | - | - | ||||
| illumina-V12S-U_REV | 18 | 559 | Vertebrates | - | - | ||||
| illumina-V12S-U_FWD | 18 | 560 | Vertebrates | - | - | ||||
| illumina-VCOI-U_REV | 18 | 561 | Vertebrates | - | - | ||||
| illumina-VCOI-U_FWD | 18 | 562 | Vertebrates | - | - | ||||
| illumina-Anisakidae_16s_F | 18 | 563 | Nematodes | - | - | ||||
| illumina-Anisakidae_16s_R | 18 | 564 | Nematodes | - | - | ||||
| illumina-MiDeca F | 18 | 565 | Decapods | - | - | ||||
| illumina-MiDeca R | 18 | 566 | Decapods | - | - | ||||
| psyl2_FWD | 18 | 567 | - | ||||||
| psyl2_REV | 18 | 568 | - | ||||||
| psyl16_FWD | 18 | 569 | - | ||||||
| psyl16_REV | 19 | 570 | - | ||||||
| psyl17_FWD | 19 | 571 | - | ||||||
| psyl17_REV | 19 | 572 | - | ||||||
| psyl25_FWD | 19 | 573 | - | ||||||
| psyl25_REV | 19 | 574 | - | ||||||
| psyl42_FWD | 19 | 575 | - | ||||||
| psyl42_REV | 19 | 576 | - | ||||||
| psyl57_FWD | 19 | 577 | - | ||||||
| psyl57_REV | 19 | 578 | - | ||||||
| SPAC11.4_FWD | 19 | 579 | - | ||||||
| SPAC11.4_REV | 19 | 580 | - | ||||||
| SPAG11.8_FWD | 19 | 581 | - | ||||||
| SPAG11.8_REV | 19 | 582 | - | ||||||
| SPAC12.5_FWD | 19 | 583 | - | ||||||
| SPAC12.5_REV | 19 | 584 | - | ||||||
| spag7.14_FWD | 19 | 585 | - | ||||||
| spag7.14_REV | 19 | 586 | - | ||||||
| PtTX4001a_FWD | 19 | 587 | - | ||||||
| PtTX4001a_REV | 19 | 588 | - | ||||||
| pTctg4363_FWD | 19 | 589 | - | ||||||
| pTctg4363_REV | 19 | 590 | - | ||||||
| TheoSF1 | 19 | 591 | Theodoxus | - | |||||
| TheoSR1 | 19 | 592 | Theodoxus | - | |||||
| Lm_521_FWD | 19 | 593 | Lisotriton vulgaris | - | |||||
| Lm_521_REV | 19 | 594 | Lisotriton vulgaris | - | |||||
| LVG_210_FWD | 19 | 595 | Lisotriton vulgaris | - | |||||
| LVG_210_REV | 19 | 596 | Lisotriton vulgaris | - | |||||
| LVG_388_FWD | 20 | 597 | Lisotriton vulgaris | - | |||||
| LVG_388_REV | 20 | 598 | Lisotriton vulgaris | - | |||||
| LVG_542_FWD | 20 | 599 | Lisotriton vulgaris | - | |||||
| LVG_542_REV | 20 | 600 | Lisotriton vulgaris | - | |||||
| Lm_488_FWD | 20 | 601 | Lisotriton vulgaris | - | |||||
| Lm_488_REV | 20 | 602 | Lisotriton vulgaris | - | |||||
| Lm_632_FWD | 20 | 603 | Lisotriton vulgaris | - | |||||
| Lm_632_REV | 20 | 604 | Lisotriton vulgaris | - | |||||
| Th_09_FWD | 20 | 605 | Lisotriton vulgaris | - | |||||
| Th_09_REV | 20 | 606 | Lisotriton vulgaris | - | |||||
| Tv3Ca9_FWD | 20 | 607 | Lisotriton vulgaris | - | |||||
| Tv3Ca9_REV | 20 | 608 | Lisotriton vulgaris | - | |||||
| Lm_013_FWD | 20 | 609 | Lisotriton vulgaris | - | |||||
| Lm_013_REV | 20 | 610 | Lisotriton vulgaris | - | |||||
| Lm_749_FWD | 20 | 611 | Lisotriton vulgaris | - | |||||
| Lm_749_REV | 20 | 612 | Lisotriton vulgaris | - | |||||
| LVG_398_FWD | 20 | 613 | Lisotriton vulgaris | - | |||||
| LVG_398_REV | 20 | 614 | Lisotriton vulgaris | - | |||||
| Tv3Ca19_FWD | 20 | 615 | Lisotriton vulgaris | - | |||||
| Tv3Ca19_REV | 20 | 616 | Lisotriton vulgaris | - | |||||
| P5 | 20 | 617 | CO1 Insects (IBA) | - | |||||
| P5 ins1 | 20 | 618 | CO1 Insects (IBA) | - | |||||
| P5 ins 2 | 20 | 619 | CO1 Insects (IBA) | - | |||||
| P5 ins 3 | 20 | 620 | CO1 Insects (IBA) | - | |||||
| P7 | 20 | 621 | CO1 Insects (IBA) | - | |||||
| P7 ins 1 | 20 | 622 | CO1 Insects (IBA) | - | |||||
| P7 ins 2 | 20 | 623 | CO1 Insects (IBA) | - | |||||
| P7 sub 1 | 20 | 624 | CO1 Insects (IBA) | - | |||||
| H54 | 21 | 625 | ??? | - | |||||
| L16245 | 21 | 626 | ??? | - | |||||
| A(L-14995) | 21 | 627 | ??? | - | |||||
| igsF-tag1 | 21 | 628 | ??? | - | |||||
| igsF-tag2 | 21 | 629 | ??? | - | |||||
| igsF-tag3 | 21 | 630 | ??? | - | |||||
| igsR-tag1 | 21 | 631 | ??? | - | |||||
| igsR-tag2 | 21 | 632 | ??? | - | |||||
| igsR-tag3 | 21 | 633 | ??? | - | |||||
| FIB-BI7U | 21 | 634 | ??? | selfmade | - | ||||
| FIB-BI7L | 21 | 635 | ??? | selfmade | - | ||||
| Panth_pers_CO1_FWD | 21 | 636 | Panthera persica | selfmade | - | ||||
| Panth_pers_CO1_REV | 21 | 637 | Panthera persica | selfmade | - | ||||
| illumina_mICO1intF | 21 | 638 | Metazoa (marine inverts) | - | Leray et al 2013 A new versatile primer set targeting a short fragment of the mitochondrial COI region for metabarcoding metazoan diversity: application for characterizing coral reef fish gut contents | 5′-GGWACWRGWTGRACWNTNTAYCCYCC-3 | |||
| illumina_rgHCO2198 | 21 | 639 | Metazoa (marine inverts) | - | Geller et al 2013 Redesign of PCR primers for mitochondrial cytochrome coxidase subunit I for marine invertebrates and application inall-taxa biotic surveys | 5′-TANACYTCNGGRTGNCCRAARAAYCA-3′ | |||
| illumina-ITS4 | 21 | 640 | Plants | - | |||||
| illumina-ITS5 | 21 | 641 | Plants | - | |||||
| illumina-RbcL_F | 21 | 642 | Plants | - | |||||
| illumina-RbcL_R | 21 | 643 | Plants | - | |||||
| illumina-BR3 | 21 | 644 | Insects | - | |||||
| ZFX923 | 21 | 645 | Marine mammals | - | Bérubé and Palsbøll 1996 | ||||
| ZFY767 | 21 | 646 | Marine mammals | - | Bérubé and Palsbøll 1996 | ||||
| ZFXY582 | 21 | 647 | Marine mammals | - | Bérubé and Palsbøll 1996 | ||||
| 3F_P_leo | 21 | 648 | Lion | - | |||||
| 3R_P_leo | 21 | 649 | Lion | - |
LepF1 LepR1 finns i Låda 3 Insect primers och i Låda 4 (DNA labbets)
| Name | Box | Number | Species | Type | Loci | Modification | Reference | Sequence |
|---|---|---|---|---|---|---|---|---|
| Rana_arvalis_cytb | Tagman 1 | T1 | Rana arvalis | qPCR | ||||
| Rana_temporaria_cytb | Tagman 1 | T2 | Rana temporaria | qPCR | ||||
| LVCB (Mindre vattensalamander) | Tagman 1 | T3 | Lissotron vulgaris | qPCR | ||||
| PFCB (lökgroda) | Tagman 1 | T4 | Pelobatus fuscus | qPCR | ||||
| Astast_CO1_P0357 (Flodkräfta | Tagman 1 | T5 | Astacus astacus | qPCR | ||||
| Bufo_calamita_16s | Tagman 1 | T6 | Bufo calamita | qPCR | ||||
| Paclen_CO1_P0357 (signalkräfta) | Tagman 1 | T7 | Pacifasticus leniusculus | qPCR | ||||
| TCCB (Större vattensalamander) | Tagman 1 | T8 | Triturus cristatus | qPCR | ||||
| Bufo_bufo_cyb | Tagman 1 | T9 | Bufo bufo | qPCR | ||||
| Cottus_gobio | Tagman 2 | T10 | Cottus gobio | qPCR | ||||
| P_phoxinus | Tagman 2 | T11 | Phoxinus phoxinus | qPCR | ||||
| S_salar | Tagman 2 | T12 | Salma salar | qPCR | ||||
| Salmo_trutta (Öring) | Tagman 2 | T13 | Salmo trutta | qPCR | ||||
| Astacus_astacus_CO1 | Tagman 2 | T14 | Astacus astacus | qPCR | ||||
| Unio_crassus_M | Tagman 2 | T15 | Unio crassus | qPCR | ||||
| Unio_crassus_F | Tagman 2 | T16 | Unio crassus | qPCR | ||||
| Unio_pictorum_CO1 | Tagman 2 | T17 | Unio pictorum | qPCR | ||||
| Pseudan_compl_16s | Tagman 2 | T18 | Pseudanodonta complanata | qPCR | ||||
| Hirudo (blodigel) | Tagman 3 | T19 | Hirudo medicinalis | qPCR | ||||
| LpCO1b (Citronfläckad kärrtrollslända) | Tagman 3 | T20 | Leucorrhinia pectoralis | qPCR | ||||
| Aspius | Tagman 3 | T21 | Leuciscius aspius | qPCR | ||||
| DytlatP (Dytiscus lattisumus) | Tagman 3 | T22 | Dytiscus lattisimus | qPCR | ||||
| Wolverine | Tagman 4 | T28 | Gulo gulo | qPCR | ||||
| Siluris glansis | Tagman 4 | T29 | Silurus glanis | qPCR | ||||
| El_cytb (Esox Lucius) | Tagman 4 | T30 | Esox lucius | qPCR | ||||
| Nitella mucronata | Tagman 4 | T31 | Nitella mucronata | qPCR | ||||
| M_margaritifera | Tagman 4 | T32 | Margaritifera margaritifera | qPCR | ||||
| PMT1 Tobacco | Tagman 4 | T33 | Tobacco | qPCR | ||||
| RBTF (Regnbåge) | Tagman 4 | T34 | Oncorhynchus mykiss | qPCR | ||||
| Hyla arborea | Tagman 4 | T35 | Hyla arborea | qPCR | ||||
| V_lagopus | Tagman 5 | T35.2 | Vulpes lagopus | qPCR | ||||
| V_vulpes | Tagman 5 | T36 | Vulpes vulpes | qPCR | ||||
| Rana_dalmatina | Tagman 5 | T37 | Rana dalmatina | qPCR | ||||
| B_viridis | Tagman 5 | T38 | Buxbaumia viridis | qPCR | ||||
| Tolypella_ITS2 (Fjällrufse) - DON’T USE | Tagman 5 | T39 | Tolypella canadensis | qPCR | ||||
| Thymallus (Harr) | Tagman 5 | T40 | Thymallus thymallus | qPCR | ||||
| E_nuuttalli | Tagman 5 | T42 | Elodea nuttalli | qPCR | ||||
| E_canadensis | Tagman 5 | T43 | Elodea canadensis | qPCR | ||||
| GobyCOI (Svartmunnad Smörbult) | Tagman 6 | T44 | Neogobius melanostomus | qPCR | ||||
| Lampetra | Tagman 6 | T45 | Lampetra | qPCR | ||||
| Dp_P | Tagman 6 | T46 | Dreissena polymorpha | qPCR | ||||
| Rangia_cunatea | Tagman 6 | T47 | Rangia cunatea | qPCR | ||||
| Triops (Hästskoräka) | Tagman 6 | T48 | Triops cancriformis | qPCR | ||||
| P_friesii_psbA | Tagman 6 | T49 | Potamogeton friesii | qPCR | ||||
| ITS_Tolypella_v3 (Fjällrufse) - DON’T USE. Use v4 | Tagman 6 | T50 | Tolypella canadensis | qPCR | ||||
| ITS_Tolypella_v4 (Fjällrufse) | Tagman 6 | T51 | Tolypella canadensis | qPCR | ||||
| SSUDF | Tagman 6 | T52 | Sarcoptes scabiei | qPCR | ||||
| Salfon-A/S715 (Bäckröding) | Tagman 7 | T53 | Salvelinus fontalis | qPCR | ||||
| SalvAlp (Fjällröding) | Tagman 7 | T54 | Salvelinus alpinus | qPCR | ||||
| CruCarp-CyTritB | Tagman 7 | T56 | Carassius carassius | qPCR | ||||
| Rhihar_co1_po3 | Tagman 7 | T57 | Rhithropanopeus harrisii | qPCR | ||||
| Limnadia_lenticularis | Tagman 7 | T58 | Limnadia lenticularis | qPCR | ||||
| LACOI | Tagman 7 | T59 | Lepidurus apus | qPCR |
| Name | Box | Number | Species | Type | Loci | Modification | Reference | Sequence |
|---|
| Name | Box | Number | Species | Type | Loci | Modification | Reference | Sequence |
| G10L_Illumina | Bear Illumina Primers | I1 | Bear | NGS | Adapters for Illumina | |||
| MU05_Illumina | Bear Illumina Primers | I2 | Bear | NGS | Adapters for Illumina | |||
| MU59_Illumina | Bear Illumina Primers | I3 | Bear | NGS | Adapters for Illumina | |||
| MU09_Illumina | Bear Illumina Primers | I4 | Bear | NGS | Adapters for Illumina | |||
| MU23_Illumina | Bear Illumina Primers | I5 | Bear | NGS | Adapters for Illumina | |||
| MU51_Illumina | Bear Illumina Primers | I6 | Bear | NGS | Adapters for Illumina | |||
| MU50_Illumina | Bear Illumina Primers | I7 | Bear | NGS | Adapters for Illumina | |||
| MU10_Illumina | Bear Illumina Primers | I8 | Bear | NGS | Adapters for Illumina | |||
| G10B_Illumina | Bear Illumina Primers | I9 | Bear | NGS | Adapters for Illumina | |||
| G1A_Illumina | Bear Illumina Primers | I10 | Bear | NGS | Adapters for Illumina | |||
| G1D_Illumina | Bear Illumina Primers | I11 | Bear | NGS | Adapters for Illumina | |||
| MU15_Illumina | Bear Illumina Primers | I12 | Bear | NGS | Adapters for Illumina |
| Name | Box | Number | Species | Type | Loci | Modification | Reference | Sequence |
| Ua_snp_01y_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_01y_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_02y_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_02y_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_03y_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_03y_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_04y_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_04y_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_03mt_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_03mt_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_04mt_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_04mt_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_05mt_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_05mt_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_07mt_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_07mt_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_102_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_102_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_104_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_104_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_105_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_105_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_111_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_111_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_112_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_112_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_114_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_114_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_115_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_115_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_116_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_116_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_118_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_118_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_119_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_119_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_120_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_120_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_125_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_125_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_127_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_127_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_128_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_128_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_129_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_129_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_131_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_131_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_133_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_133_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_134_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_134_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_136_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_136_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_141_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_141_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_145_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_145_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_146_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_146_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_147_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_147_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_150_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_150_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_156_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_156_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_159_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_159_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_160_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_160_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_162_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_162_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_164_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_164_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_165_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_165_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_166_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_166_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_168_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_168_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_169_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_169_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_170_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_170_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_172_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_172_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_175_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_175_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_176_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_176_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_177_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_177_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_179_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_179_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_180_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_180_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_181_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_181_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_183_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_183_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_184_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_184_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_186_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_186_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_189_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_189_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_191_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_191_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_193_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_193_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_195_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_195_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_196-x_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_196-x_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_199_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_199_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_200_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_200_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_201_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_201_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_202_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_202_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_203_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_203_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_204_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_204_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_205_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_205_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_206_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_206_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_207_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_207_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_209_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_209_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_211_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_211_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_212_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_212_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_213_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_213_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_214_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_214_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_217_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_217_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_218_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_218_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_219_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_219_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_220_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_220_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_221_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_221_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_222_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_222_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_223_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_223_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_225_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_225_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_226_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_226_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_227_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_227_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_228_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_228_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_230_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_230_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_231_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_231_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_234_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_234_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_236-x_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_236-x_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_237_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_237_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_239_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_239_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_240_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_240_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_241_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_241_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_244_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_244_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_245_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_245_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_248-x_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_248-x_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_250_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_250_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_251_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_251_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_253_LSP | Bear SNP Primers | Bear | NGS | Adapters for Illumina | ||||
| Ua_snp_253_STA | Bear SNP Primers | Bear | NGS | Adapters for Illumina |
For most of the qPCR reactions we are running a simple standard protocol. We run on a CFX96 Touch™ Real-Time PCR Detection System from BIO-RAD. For standard eDNA water samples we run between 3 and 6 technical replicates. Fish species is often more stable and there is no need to run large number of replicates. but insects and many organism that are more sporadically found in the water, tend to require more replicates to be able to get high quality data.
The standard amplification procedures for either sanger sequencing or other applications we use Illustra Ready-to-go PCR beads. For more complicated PCR reactions that require high fidelity PCR products we use Phusion enzymes