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How to use microeco to calculate humann results #445

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makerer5 opened this issue Dec 27, 2024 · 1 comment
Open

How to use microeco to calculate humann results #445

makerer5 opened this issue Dec 27, 2024 · 1 comment
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documentation Improvements or additions to documentation

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@makerer5
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Hi Chi
The microeco package has a very clear and smooth visualization process for amplicon data. I previously used the microeco package you developed to complete the publication of the corresponding paper and cited "microeco" in the paper.
However, I am currently encountering such a problem. When I try to continue using "microeco" to process the files generated by humann3, I find that "Chapter 8.3 in the microeco tutorial" seems to only be about the correlation analysis of metabolic pathways. I tried to use the species abundance file (metaphlan4--taxonomy.tsv) generated by humann3 instead of "metacyc.tsv", but I was unable to build a microeco object.
So, my question is: Does the "microeco tutorial" contain a tutorial for correlation analysis of humann results, so that metagenome results can also calculate: α/β diversity, co-occurrence networks, differential species, environmental factors, etc.?

Wish you all the best

@ChiLiubio
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Hi. Yes. The basic processes for metagenomic data are similar with the analysis steps for amplicon data, as the data structures are similar in two types of microtable objects. Therefore, I only included a few unique examples in the tutorial. Please feel free to tell me if you encounter any error or confused issue. For metaphlan4 data, please use mpa2meco function in file2meco package.

Best,
Chi

@ChiLiubio ChiLiubio added the documentation Improvements or additions to documentation label Dec 27, 2024
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