8.8 PICRUSt2

[jimena18-O]

Hello, I was able to run the FAPROTAX analysis with my data and now I would like to run the PICRUST2 (Tax4Fun2_KEGG) analysis. How can I do this? I would like to make the same graph of differential relative abundances.

select the "func" in taxa_abund list in trans_diff

t2 <- trans_diff$new(dataset = tmp_mt, method = "anova", group = "Group", taxa_level = "func")
t2$plot_diff_abund(add_sig = T) + ggplot2::ylab("Relative abundance (%)")

That graph but with the KEGG analysis

Thanks!!

[ChiLiubio]

Hi. Please check the tutorial for the example of differential test (https://chiliubio.github.io/microeco_tutorial/explainable-class.html#trans_func-class). It is similar with the operation of amplicon data. For the data input, please refer to the file2meco chapter (https://chiliubio.github.io/microeco_tutorial/file2meco-package.html#picrust2).