Simulating a nucleoprotein using MARTINI_v2

[satyajitkhatua09]

I have a nucleoprotein that was parametrized with the Martinize Python scripts for protein and DNA (as in Advanced tutorial: protein-DNA complex](https://cgmartini.nl/docs/tutorials/Legacy/martini2/dna.html). But while running the simulations, It stuck in the equilibration step with the error log attached. The system with protein only is running okay.

As this is the first time I am using CG simulation and also using GROMACS MD engine, I find it very difficult to solve the errors. The following is the equilibration input:

integrator = md
dt = 0.010
nsteps = 200000

nstxout = 0
nstvout = 0
nstfout = 0
nstlog = 1000
nstenergy = 100
nstxout-compressed = 1000
compressed-x-precision = 100

cutoff-scheme = Verlet
nstlist = 10
ns_type = grid
pbc = xyz
verlet-buffer-tolerance = 0.005

coulombtype = reaction-field
rcoulomb = 1.1
epsilon_r = 15
vdw_type = cutoff
vdw-modifier = Potential-shift-verlet
rvdw = 1.1

tcoupl = v-rescale
tc-grps = System
tau_t = 2.0 ;0.5
ref_t = 300
Pcoupltype = isotropic
Pcoupl = berendsen
tau_p = 3.0
compressibility = 3e-4
ref_p = 1.0
refcoord-scaling = com

constraints = none

equil.log

[cgmartini]

Your initial temperature seems to be very low (close to 0 K). Better to provide initial velocities (gen_vel = yes).