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config.toml
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usage = "test" #["build", "target", "classify", "test"]
output_directory = 'RB_out'
log_directory = 'RB_out/logs'
[IBF]
kmer_size = 15 #(unsigned integer with default 13) only required for 'usage = "build"' or if target_file/deplete_file is a fasta formate file
fragment_size = 100000 #(unsigned integer with default 100000) only required for 'usage = "build" or if target_file/deplete_file is a fasta formate file
threads = 3 #(unsigned integer with default 3)
target_files = ['path/to/reference/file/Bacillus_subtilis_complete_genome.fasta', 'path/to/reference/file/Enterococcus_faecalis_complete_genome.fasta', 'path/to/reference/file/Escherichia_coli_complete_genome.fasta']
deplete_files = ['path/to/reference/file/Saccharomyces_cerevisiae_draft_genome.fasta']
read_files = ['path/to/read/file/SampleZMCDataSet.fasta']
exp_seq_error_rate = 0.1 #(unsigned float between 0 and 1 default 0.1)
chunk_length = 360 #(unsigned integer with default 250)
max_chunks = 1 #(unsigned integer with default 5)
[MinKNOW]
host = "localhost" #(ip address or name of the computer hosting MinKNOW)
port = "9502" #(port number used fo grpc communication by by MinKNOW instance)
flowcell = "MS00000" #(name of the flowcell used)
token_path = "test/tmp/minknow-auth-token.json" #path/to/authentication/token/path (remote file)
channels = [1,512]
[Basecaller]
caller = "DeepNano" #DeepNano/Guppy (default is DeepNano)
host = "127.0.0.1"#(ip address or name of the computer hosting Guppy Basecall Server)
port = "9502" #(port number on which the basecall server is running on the host)
threads = 3 # (unsigned integer with default 3)
config = "dna_r9.4.1_450bps_fast"