diff --git a/.changes/next-release/enhancement-crt-30257.json b/.changes/next-release/enhancement-crt-30257.json
new file mode 100644
index 00000000..dca8d87d
--- /dev/null
+++ b/.changes/next-release/enhancement-crt-30257.json
@@ -0,0 +1,5 @@
+{
+  "type": "enhancement",
+  "category": "``crt``",
+  "description": "Automatically configure CRC32 checksums for uploads and checksum validation for downloads through the CRT transfer manager."
+}
diff --git a/s3transfer/crt.py b/s3transfer/crt.py
index eda2985f..ed5a0864 100644
--- a/s3transfer/crt.py
+++ b/s3transfer/crt.py
@@ -206,6 +206,7 @@ def upload(self, fileobj, bucket, key, extra_args=None, subscribers=None):
             extra_args = {}
         if subscribers is None:
             subscribers = {}
+        self._validate_checksum_algorithm_supported(extra_args)
         callargs = CallArgs(
             bucket=bucket,
             key=key,
@@ -231,6 +232,17 @@ def delete(self, bucket, key, extra_args=None, subscribers=None):
     def shutdown(self, cancel=False):
         self._shutdown(cancel)
 
+    def _validate_checksum_algorithm_supported(self, extra_args):
+        checksum_algorithm = extra_args.get('ChecksumAlgorithm')
+        if checksum_algorithm is None:
+            return
+        supported_algorithms = list(awscrt.s3.S3ChecksumAlgorithm.__members__)
+        if checksum_algorithm.upper() not in supported_algorithms:
+            raise ValueError(
+                f'ChecksumAlgorithm: {checksum_algorithm} not supported. '
+                f'Supported algorithms are: {supported_algorithms}'
+            )
+
     def _cancel_transfers(self):
         for coordinator in self._future_coordinators:
             if not coordinator.done():
@@ -623,11 +635,17 @@ def _get_make_request_args_put_object(
         else:
             call_args.extra_args["Body"] = call_args.fileobj
 
+        checksum_algorithm = call_args.extra_args.pop(
+            'ChecksumAlgorithm', 'CRC32'
+        ).upper()
+        checksum_config = awscrt.s3.S3ChecksumConfig(
+            algorithm=awscrt.s3.S3ChecksumAlgorithm[checksum_algorithm],
+            location=awscrt.s3.S3ChecksumLocation.TRAILER,
+        )
         # Suppress botocore's automatic MD5 calculation by setting an override
         # value that will get deleted in the BotocoreCRTRequestSerializer.
-        # The CRT S3 client is able automatically compute checksums as part of
-        # requests it makes, and the intention is to configure automatic
-        # checksums in a future update.
+        # As part of the CRT S3 request, we request the CRT S3 client to
+        # automatically add trailing checksums to its uploads.
         call_args.extra_args["ContentMD5"] = "override-to-be-removed"
 
         make_request_args = self._default_get_make_request_args(
@@ -639,6 +657,7 @@ def _get_make_request_args_put_object(
             on_done_after_calls=on_done_after_calls,
         )
         make_request_args['send_filepath'] = send_filepath
+        make_request_args['checksum_config'] = checksum_config
         return make_request_args
 
     def _get_make_request_args_get_object(
@@ -652,6 +671,7 @@ def _get_make_request_args_get_object(
     ):
         recv_filepath = None
         on_body = None
+        checksum_config = awscrt.s3.S3ChecksumConfig(validate_response=True)
         if isinstance(call_args.fileobj, str):
             final_filepath = call_args.fileobj
             recv_filepath = self._os_utils.get_temp_filename(final_filepath)
@@ -673,6 +693,7 @@ def _get_make_request_args_get_object(
         )
         make_request_args['recv_filepath'] = recv_filepath
         make_request_args['on_body'] = on_body
+        make_request_args['checksum_config'] = checksum_config
         return make_request_args
 
     def _default_get_make_request_args(
diff --git a/tests/functional/test_crt.py b/tests/functional/test_crt.py
index 152949d2..c56ea301 100644
--- a/tests/functional/test_crt.py
+++ b/tests/functional/test_crt.py
@@ -128,9 +128,11 @@ def _assert_expected_crt_http_request(
                 str(expected_content_length),
             )
         if expected_missing_headers is not None:
-            header_names = [header[0] for header in crt_http_request.headers]
+            header_names = [
+                header[0].lower() for header in crt_http_request.headers
+            ]
             for expected_missing_header in expected_missing_headers:
-                self.assertNotIn(expected_missing_header, header_names)
+                self.assertNotIn(expected_missing_header.lower(), header_names)
 
     def _assert_subscribers_called(self, expected_future=None):
         self.assertTrue(self.record_subscriber.on_queued_called)
@@ -143,6 +145,21 @@ def _assert_subscribers_called(self, expected_future=None):
                 self.record_subscriber.on_done_future, expected_future
             )
 
+    def _get_expected_upload_checksum_config(self, **overrides):
+        checksum_config_kwargs = {
+            'algorithm': awscrt.s3.S3ChecksumAlgorithm.CRC32,
+            'location': awscrt.s3.S3ChecksumLocation.TRAILER,
+        }
+        checksum_config_kwargs.update(overrides)
+        return awscrt.s3.S3ChecksumConfig(**checksum_config_kwargs)
+
+    def _get_expected_download_checksum_config(self, **overrides):
+        checksum_config_kwargs = {
+            'validate_response': True,
+        }
+        checksum_config_kwargs.update(overrides)
+        return awscrt.s3.S3ChecksumConfig(**checksum_config_kwargs)
+
     def _invoke_done_callbacks(self, **kwargs):
         callargs = self.s3_crt_client.make_request.call_args
         callargs_kwargs = callargs[1]
@@ -180,6 +197,7 @@ def test_upload(self):
                 'send_filepath': self.filename,
                 'on_progress': mock.ANY,
                 'on_done': mock.ANY,
+                'checksum_config': self._get_expected_upload_checksum_config(),
             },
         )
         self._assert_expected_crt_http_request(
@@ -206,6 +224,7 @@ def test_upload_from_seekable_stream(self):
                     'send_filepath': None,
                     'on_progress': mock.ANY,
                     'on_done': mock.ANY,
+                    'checksum_config': self._get_expected_upload_checksum_config(),
                 },
             )
             self._assert_expected_crt_http_request(
@@ -237,6 +256,7 @@ def test_upload_from_nonseekable_stream(self):
                 'send_filepath': None,
                 'on_progress': mock.ANY,
                 'on_done': mock.ANY,
+                'checksum_config': self._get_expected_upload_checksum_config(),
             },
         )
         self._assert_expected_crt_http_request(
@@ -251,6 +271,90 @@ def test_upload_from_nonseekable_stream(self):
         )
         self._assert_subscribers_called(future)
 
+    def test_upload_override_checksum_algorithm(self):
+        future = self.transfer_manager.upload(
+            self.filename,
+            self.bucket,
+            self.key,
+            {'ChecksumAlgorithm': 'CRC32C'},
+            [self.record_subscriber],
+        )
+        future.result()
+
+        callargs_kwargs = self.s3_crt_client.make_request.call_args[1]
+        self.assertEqual(
+            callargs_kwargs,
+            {
+                'request': mock.ANY,
+                'type': awscrt.s3.S3RequestType.PUT_OBJECT,
+                'send_filepath': self.filename,
+                'on_progress': mock.ANY,
+                'on_done': mock.ANY,
+                'checksum_config': self._get_expected_upload_checksum_config(
+                    algorithm=awscrt.s3.S3ChecksumAlgorithm.CRC32C
+                ),
+            },
+        )
+        self._assert_expected_crt_http_request(
+            callargs_kwargs["request"],
+            expected_http_method='PUT',
+            expected_content_length=len(self.expected_content),
+            expected_missing_headers=[
+                'Content-MD5',
+                'x-amz-sdk-checksum-algorithm',
+                'X-Amz-Trailer',
+            ],
+        )
+        self._assert_subscribers_called(future)
+
+    def test_upload_override_checksum_algorithm_accepts_lowercase(self):
+        future = self.transfer_manager.upload(
+            self.filename,
+            self.bucket,
+            self.key,
+            {'ChecksumAlgorithm': 'crc32c'},
+            [self.record_subscriber],
+        )
+        future.result()
+
+        callargs_kwargs = self.s3_crt_client.make_request.call_args[1]
+        self.assertEqual(
+            callargs_kwargs,
+            {
+                'request': mock.ANY,
+                'type': awscrt.s3.S3RequestType.PUT_OBJECT,
+                'send_filepath': self.filename,
+                'on_progress': mock.ANY,
+                'on_done': mock.ANY,
+                'checksum_config': self._get_expected_upload_checksum_config(
+                    algorithm=awscrt.s3.S3ChecksumAlgorithm.CRC32C
+                ),
+            },
+        )
+        self._assert_expected_crt_http_request(
+            callargs_kwargs["request"],
+            expected_http_method='PUT',
+            expected_content_length=len(self.expected_content),
+            expected_missing_headers=[
+                'Content-MD5',
+                'x-amz-sdk-checksum-algorithm',
+                'X-Amz-Trailer',
+            ],
+        )
+        self._assert_subscribers_called(future)
+
+    def test_upload_throws_error_for_unsupported_checksum(self):
+        with self.assertRaisesRegex(
+            ValueError, 'ChecksumAlgorithm: UNSUPPORTED not supported'
+        ):
+            self.transfer_manager.upload(
+                self.filename,
+                self.bucket,
+                self.key,
+                {'ChecksumAlgorithm': 'UNSUPPORTED'},
+                [self.record_subscriber],
+            )
+
     def test_download(self):
         future = self.transfer_manager.download(
             self.bucket, self.key, self.filename, {}, [self.record_subscriber]
@@ -267,6 +371,7 @@ def test_download(self):
                 'on_progress': mock.ANY,
                 'on_done': mock.ANY,
                 'on_body': None,
+                'checksum_config': self._get_expected_download_checksum_config(),
             },
         )
         # the recv_filepath will be set to a temporary file path with some
@@ -304,6 +409,7 @@ def test_download_to_seekable_stream(self):
                 'on_progress': mock.ANY,
                 'on_done': mock.ANY,
                 'on_body': mock.ANY,
+                'checksum_config': self._get_expected_download_checksum_config(),
             },
         )
         self._assert_expected_crt_http_request(
@@ -338,6 +444,7 @@ def test_download_to_nonseekable_stream(self):
                 'on_progress': mock.ANY,
                 'on_done': mock.ANY,
                 'on_body': mock.ANY,
+                'checksum_config': self._get_expected_download_checksum_config(),
             },
         )
         self._assert_expected_crt_http_request(