diff --git a/e2e/components/Toggletip/Toggletip-test.avt.e2e.js b/e2e/components/Toggletip/Toggletip-test.avt.e2e.js
new file mode 100644
index 000000000000..26525fc2519b
--- /dev/null
+++ b/e2e/components/Toggletip/Toggletip-test.avt.e2e.js
@@ -0,0 +1,57 @@
+/**
+ * Copyright IBM Corp. 2016, 2023
+ *
+ * This source code is licensed under the Apache-2.0 license found in the
+ * LICENSE file in the root directory of this source tree.
+ */
+
+'use strict';
+
+import { expect, test } from '@playwright/test';
+import { visitStory } from '../../test-utils/storybook';
+
+test.describe('Toggletip @avt', () => {
+  test('@avt-default-state Toggletip', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Toggletip',
+      id: 'components-toggletip--default',
+      globals: {
+        theme: 'white',
+      },
+    });
+    await expect(page).toHaveNoACViolations('Toggletip');
+  });
+
+  test('@avt-keyboard-nav Toggletip', async ({ page }) => {
+    await visitStory(page, {
+      component: 'Toggletip',
+      id: 'components-toggletip--default',
+      globals: {
+        theme: 'white',
+      },
+    });
+
+    // Checking if the defaultOpen is working
+    await expect(page.locator('.cds--popover--open')).toBeVisible();
+
+    // Checking first Toggletip interaction
+    await page.keyboard.press('Tab');
+    await expect(page.getByLabel('Show information').first()).toBeFocused();
+    await page.keyboard.press('Enter');
+    await expect(page.locator('.cds--popover--open')).toBeVisible();
+    // Tabbing inside the popover
+    await page.keyboard.press('Tab');
+    await expect(page.locator('.cds--link').first()).toBeFocused();
+    await page.keyboard.press('Tab');
+    await expect(page.getByRole('button', { name: 'Button' })).toBeFocused();
+    await page.keyboard.press('Tab');
+    await expect(page.locator('.cds--popover--open')).not.toBeVisible();
+
+    // Checking second Toggletip interaction and close on Escape key
+    await expect(page.getByLabel('Show information').last()).toBeFocused();
+    await page.keyboard.press('Enter');
+    await expect(page.locator('.cds--popover--open')).toBeVisible();
+    await page.keyboard.press('Escape');
+    await expect(page.locator('.cds--popover--open')).not.toBeVisible();
+  });
+});
diff --git a/e2e/components/Toggletip/Toggletip-test.e2e.js b/e2e/components/Toggletip/Toggletip-test.e2e.js
index df57ebbc70fb..b64f2a196073 100644
--- a/e2e/components/Toggletip/Toggletip-test.e2e.js
+++ b/e2e/components/Toggletip/Toggletip-test.e2e.js
@@ -7,9 +7,9 @@
 
 'use strict';
 
-const { expect, test } = require('@playwright/test');
-const { themes } = require('../../test-utils/env');
-const { snapshotStory, visitStory } = require('../../test-utils/storybook');
+import { test } from '@playwright/test';
+import { themes } from '../../test-utils/env';
+import { snapshotStory } from '../../test-utils/storybook';
 
 test.describe('Toggletip', () => {
   themes.forEach((theme) => {
@@ -23,15 +23,4 @@ test.describe('Toggletip', () => {
       });
     });
   });
-
-  test('accessibility-checker @avt', async ({ page }) => {
-    await visitStory(page, {
-      component: 'Toggletip',
-      id: 'components-toggletip--default',
-      globals: {
-        theme: 'white',
-      },
-    });
-    await expect(page).toHaveNoACViolations('Toggletip');
-  });
 });