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Dear all, I'm rather new to scRNA-seq analysis and am even newer on the program DoubletFinder. I'm following the tutorial to run it on R. I got stuck when I read the Data section (under required), which stated:
" Expected number of doublets ($DOUBLETS)
This can be calculated based on the number of droplets captured using our [Doublet Estimation Calculator]
I went onto the [Doublet Estimation Calculator], and it asks me to put in the number of droplets captured.
I'm using the CellRanger output to create Seurat Objects. And my questions are:
How can I get the number of droplets captured?
Is it the number of cells (rows in the metadata) of the Seurat object created from the CellRanger filtered_feature_bc_matrix output?
Or is it the number of cells from the CellRanger raw_feature_bc_matrix output?
Thank you very much for your time and support!
The text was updated successfully, but these errors were encountered:
Dear all, I'm rather new to scRNA-seq analysis and am even newer on the program DoubletFinder. I'm following the tutorial to run it on R. I got stuck when I read the Data section (under required), which stated:
" Expected number of doublets ($DOUBLETS)
This can be calculated based on the number of droplets captured using our [Doublet Estimation Calculator]
I went onto the [Doublet Estimation Calculator], and it asks me to put in the number of droplets captured.
I'm using the CellRanger output to create Seurat Objects. And my questions are:
Thank you very much for your time and support!
The text was updated successfully, but these errors were encountered: