diff --git a/docs/api/app.md b/docs/api/app.md
index c3ecb552f8..ef9c2ea08d 100644
--- a/docs/api/app.md
+++ b/docs/api/app.md
@@ -573,3 +573,31 @@ Hooks is a method to return [hooks](./hooks.md) property.
 ```go title="Signature"
 func (app *App) Hooks() *Hooks
 ```
+
+## RebuildTree
+
+The RebuildTree method is designed to rebuild the route tree and enable dynamic route registration. It returns a pointer to the App instance.
+
+```go title="Signature"
+func (app *App) RebuildTree() *App
+```
+
+**Note:** Use this method with caution. It is **not** thread-safe and calling it can be very performance-intensive, so it should be used sparingly and only in development mode. Avoid using it concurrently.
+
+### Example Usage
+
+Here’s an example of how to define and register routes dynamically:
+
+```go
+app.Get("/define", func(c Ctx) error {  // Define a new route dynamically
+    app.Get("/dynamically-defined", func(c Ctx) error {  // Adding a dynamically defined route
+        return c.SendStatus(http.StatusOK)
+    })
+
+    app.RebuildTree()  // Rebuild the route tree to register the new route
+
+    return c.SendStatus(http.StatusOK)
+})
+```
+
+In this example, a new route is defined and then `RebuildTree()` is called to make sure the new route is registered and available.
diff --git a/docs/whats_new.md b/docs/whats_new.md
index 9155792f6b..aa1d05a759 100644
--- a/docs/whats_new.md
+++ b/docs/whats_new.md
@@ -389,6 +389,31 @@ app.Route("/api").Route("/user/:id?")
   });
 ```
 
+### 🗺 RebuildTree
+
+We have added a new method that allows the route tree stack to be rebuilt in runtime, with it, you can add a route while your application is running and rebuild the route tree stack to make it registered and available for calls.
+
+You can find more reference on it in the [app](./api/app.md#rebuildtree):
+
+#### Example Usage
+
+```go
+app.Get("/define", func(c Ctx) error {  // Define a new route dynamically
+    app.Get("/dynamically-defined", func(c Ctx) error {  // Adding a dynamically defined route
+        return c.SendStatus(http.StatusOK)
+    })
+
+    app.RebuildTree()  // Rebuild the route tree to register the new route
+
+    return c.SendStatus(http.StatusOK)
+})
+```
+
+In this example, a new route is defined and then `RebuildTree()` is called to make sure the new route is registered and available.
+
+**Note:** Use this method with caution. It is **not** thread-safe and calling it can be very performance-intensive, so it should be used sparingly and only in
+development mode. Avoid using it concurrently.
+
 ### 🧠 Context
 
 ### 📎 Parser
diff --git a/router.go b/router.go
index eae9adef70..bc93f67977 100644
--- a/router.go
+++ b/router.go
@@ -375,6 +375,9 @@ func (app *App) register(methods []string, pathRaw string, group *Group, handler
 }
 
 func (app *App) addRoute(method string, route *Route, isMounted ...bool) {
+	app.mutex.Lock()
+	defer app.mutex.Unlock()
+
 	// Check mounted routes
 	var mounted bool
 	if len(isMounted) > 0 {
@@ -400,15 +403,28 @@ func (app *App) addRoute(method string, route *Route, isMounted ...bool) {
 
 	// Execute onRoute hooks & change latestRoute if not adding mounted route
 	if !mounted {
-		app.mutex.Lock()
 		app.latestRoute = route
 		if err := app.hooks.executeOnRouteHooks(*route); err != nil {
 			panic(err)
 		}
-		app.mutex.Unlock()
 	}
 }
 
+// BuildTree rebuilds the prefix tree from the previously registered routes.
+// This method is useful when you want to register routes dynamically after the app has started.
+// It is not recommended to use this method on production environments because rebuilding
+// the tree is performance-intensive and not thread-safe in runtime. Since building the tree
+// is only done in the startupProcess of the app, this method does not makes sure that the
+// routeTree is being safely changed, as it would add a great deal of overhead in the request.
+// Latest benchmark results showed a degradation from 82.79 ns/op to 94.48 ns/op and can be found in:
+// https://github.com/gofiber/fiber/issues/2769#issuecomment-2227385283
+func (app *App) RebuildTree() *App {
+	app.mutex.Lock()
+	defer app.mutex.Unlock()
+
+	return app.buildTree()
+}
+
 // buildTree build the prefix tree from the previously registered routes
 func (app *App) buildTree() *App {
 	if !app.routesRefreshed {
diff --git a/router_test.go b/router_test.go
index 715fbf7661..5509039c66 100644
--- a/router_test.go
+++ b/router_test.go
@@ -9,6 +9,7 @@ import (
 	"errors"
 	"fmt"
 	"io"
+	"net/http"
 	"net/http/httptest"
 	"os"
 	"testing"
@@ -368,6 +369,33 @@ func Test_Router_NotFound_HTML_Inject(t *testing.T) {
 	require.Equal(t, "Cannot DELETE /does/not/exist<script>alert('foo');</script>", string(c.Response.Body()))
 }
 
+func Test_App_Rebuild_Tree(t *testing.T) {
+	t.Parallel()
+	app := New()
+
+	app.Get("/test", func(c Ctx) error {
+		app.Get("/dynamically-defined", func(c Ctx) error {
+			return c.SendStatus(http.StatusOK)
+		})
+
+		app.RebuildTree()
+
+		return c.SendStatus(http.StatusOK)
+	})
+
+	resp, err := app.Test(httptest.NewRequest(MethodGet, "/dynamically-defined", nil))
+	require.NoError(t, err, "app.Test(req)")
+	require.Equal(t, http.StatusNotFound, resp.StatusCode, "Status code")
+
+	resp, err = app.Test(httptest.NewRequest(MethodGet, "/test", nil))
+	require.NoError(t, err, "app.Test(req)")
+	require.Equal(t, http.StatusOK, resp.StatusCode, "Status code")
+
+	resp, err = app.Test(httptest.NewRequest(MethodGet, "/dynamically-defined", nil))
+	require.NoError(t, err, "app.Test(req)")
+	require.Equal(t, http.StatusOK, resp.StatusCode, "Status code")
+}
+
 //////////////////////////////////////////////
 ///////////////// BENCHMARKS /////////////////
 //////////////////////////////////////////////