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update main-spots-extraction
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Cristian Goina committed Oct 5, 2023
1 parent fe63079 commit 31b0a2c
Showing 1 changed file with 3 additions and 5 deletions.
8 changes: 3 additions & 5 deletions main-spots-extraction.nf
Original file line number Diff line number Diff line change
Expand Up @@ -29,8 +29,8 @@ airlocalize_params = final_params + [
airlocalize_z_overlap: airlocalize_z_overlap_param(final_params),
]
include {
airlocalize;
} from './workflows/airlocalize' addParams(airlocalize_params)
spot_extraction;
} from './workflows/spot_extraction' addParams(airlocalize_params)

channels = final_params.channels?.split(',')
bleedthrough_channels = final_params.bleed_channel?.split(',')
Expand All @@ -44,12 +44,10 @@ workflow {
log.error "Stitched image must be specified"
}

airlocalize(
spot_extraction(
final_params.stitchdir,
Channel.of(outdir),
channels,
final_params.spot_extraction_scale,
final_params.dapi_channel,
bleedthrough_channels // bleed_channel
)

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