-
Notifications
You must be signed in to change notification settings - Fork 214
Commit
This commit does not belong to any branch on this repository, and may belong to a fork outside of the repository.
Add pytest for alignment seive in sync with Planemo test
- Loading branch information
1 parent
510463e
commit 321c363
Showing
8 changed files
with
4,344 additions
and
0 deletions.
There are no files selected for viewing
This file contains bidirectional Unicode text that may be interpreted or compiled differently than what appears below. To review, open the file in an editor that reveals hidden Unicode characters.
Learn more about bidirectional Unicode characters
Original file line number | Diff line number | Diff line change |
---|---|---|
@@ -0,0 +1,83 @@ | ||
import deeptools.alignmentSieve as aln_seive | ||
|
||
import os.path | ||
from os import unlink | ||
|
||
ROOT = os.path.dirname(os.path.abspath(__file__)) + "/test_data/" | ||
BAMFILE_IN = ROOT + "paired_chr2L.bam" | ||
CRAMFILE_IN = ROOT + "paired_chr2L.cram" | ||
FILTER_METRICS_FILE = ROOT + "alignmentSieve.txt" | ||
BED_OUT = ROOT + "alignmentSieve.bed" | ||
BAMFILE_OUT = ROOT + "alignmentSieve.bam" | ||
BAMFILE_OUT2 = ROOT + "alignmentSieve2.bam" | ||
BAMFILE_OUT3 = ROOT + "alignmentSieve3.bam" | ||
|
||
|
||
def test_minimum_mapping_quality_filter_metric(): | ||
""" | ||
Test minimal command line args for alignement sieve | ||
""" | ||
outfile = '/tmp/test_bam.bam' | ||
filter_metric_file = '/tmp/test_metrics.txt' | ||
args = "--bam {} -o {} --minMappingQuality 10 --filterMetrics {}".format(BAMFILE_IN, outfile, filter_metric_file).split() | ||
aln_seive.main(args) | ||
|
||
_foo = open(filter_metric_file, "r") | ||
resp = _foo.readlines()[2] | ||
_foo.close() | ||
expected = 'paired_chr2L.bam\t8440\t12644\n' | ||
assert expected in resp, f"'{expected}' not found in '{resp}'" | ||
|
||
bam_file_size = os.path.getsize(BAMFILE_OUT) | ||
expected_file_size = os.path.getsize(outfile) | ||
size_tolerance = 500 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File size do not match" | ||
unlink(outfile) | ||
unlink(filter_metric_file) | ||
|
||
|
||
def test_with_bed_output_along_with_shifts(): | ||
""" | ||
Tests Alignment seive with shifts and output BED file | ||
""" | ||
output_bed_file = "/tmp/aln_seive.bed" | ||
args = "--bam {} -o {} --minMappingQuality 10 --BED --shift 1 -2 3 -4".format(BAMFILE_IN, output_bed_file).split() | ||
aln_seive.main(args) | ||
with open(output_bed_file, "r") as _foo: | ||
result = len(_foo.readlines()) | ||
_expected = 4261 | ||
assert result == _expected, "No of lines in BED files differ" | ||
unlink(output_bed_file) | ||
|
||
|
||
def test_with_bam_output_with_shifts(): | ||
""" | ||
Tests Alignment seive with shifts and output BAM file | ||
""" | ||
output_bam_file = "/tmp/aln_seive.bam" | ||
args = "--bam {} -o {} --minMappingQuality 10 --shift 1 -2 3 -4".format(BAMFILE_IN, output_bam_file).split() | ||
aln_seive.main(args) | ||
|
||
bam_file_size = os.path.getsize(BAMFILE_OUT2) | ||
expected_file_size = os.path.getsize(output_bam_file) | ||
size_tolerance = 1000 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File sizes do not match" | ||
unlink(output_bam_file) | ||
|
||
|
||
def test_with_cram_output_with_shifts(): | ||
""" | ||
Tests Alignment seive with CRAM input along with shifts | ||
""" | ||
output_bam_file = "/tmp/aln_seive2.bam" | ||
args = "--bam {} -o {} --minMappingQuality 10 --shift 1 -2 3 -4".format(BAMFILE_IN, output_bam_file).split() | ||
aln_seive.main(args) | ||
|
||
bam_file_size = os.path.getsize(BAMFILE_OUT3) | ||
expected_file_size = os.path.getsize(output_bam_file) | ||
size_tolerance = 1000 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File sizes do not match" | ||
unlink(output_bam_file) |
Binary file not shown.
Oops, something went wrong.