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Add pytest for alignment seive in sync with Planemo test #1364
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Original file line number | Diff line number | Diff line change |
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import deeptools.alignmentSieve as aln_seive | ||
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import os.path | ||
from os import unlink | ||
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ROOT = os.path.dirname(os.path.abspath(__file__)) + "/test_data/" | ||
BAMFILE_IN = ROOT + "paired_chr2L.bam" | ||
CRAMFILE_IN = ROOT + "paired_chr2L.cram" | ||
FILTER_METRICS_FILE = ROOT + "alignmentSieve.txt" | ||
BED_OUT = ROOT + "alignmentSieve.bed" | ||
BAMFILE_OUT = ROOT + "alignmentSieve.bam" | ||
BAMFILE_OUT2 = ROOT + "alignmentSieve2.bam" | ||
BAMFILE_OUT3 = ROOT + "alignmentSieve3.bam" | ||
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def test_minimum_mapping_quality_filter_metric(): | ||
""" | ||
Test minimal command line args for alignement sieve | ||
""" | ||
outfile = '/tmp/test_bam.bam' | ||
filter_metric_file = '/tmp/test_metrics.txt' | ||
args = "--bam {} -o {} --minMappingQuality 10 --filterMetrics {}".format(BAMFILE_IN, outfile, filter_metric_file).split() | ||
aln_seive.main(args) | ||
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_foo = open(filter_metric_file, "r") | ||
resp = _foo.readlines()[2] | ||
_foo.close() | ||
expected = 'paired_chr2L.bam\t8440\t12644\n' | ||
assert expected in resp, f"'{expected}' not found in '{resp}'" | ||
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bam_file_size = os.path.getsize(BAMFILE_OUT) | ||
expected_file_size = os.path.getsize(outfile) | ||
size_tolerance = 5000 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File size do not match" | ||
unlink(outfile) | ||
unlink(filter_metric_file) | ||
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def test_with_bed_output_along_with_shifts(): | ||
""" | ||
Tests Alignment seive with shifts and output BED file | ||
""" | ||
output_bed_file = "/tmp/aln_seive.bed" | ||
args = "--bam {} -o {} --minMappingQuality 10 --BED --shift 1 -2 3 -4".format(BAMFILE_IN, output_bed_file).split() | ||
aln_seive.main(args) | ||
with open(output_bed_file, "r") as _foo: | ||
result = len(_foo.readlines()) | ||
_expected = 4261 | ||
assert result == _expected, "No of lines in BED files differ" | ||
unlink(output_bed_file) | ||
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def test_with_bam_output_with_shifts(): | ||
""" | ||
Tests Alignment seive with shifts and output BAM file | ||
""" | ||
output_bam_file = "/tmp/aln_seive.bam" | ||
args = "--bam {} -o {} --minMappingQuality 10 --shift 1 -2 3 -4".format(BAMFILE_IN, output_bam_file).split() | ||
aln_seive.main(args) | ||
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bam_file_size = os.path.getsize(BAMFILE_OUT2) | ||
expected_file_size = os.path.getsize(output_bam_file) | ||
size_tolerance = 5000 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File sizes do not match" | ||
unlink(output_bam_file) | ||
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def test_with_cram_output_with_shifts(): | ||
""" | ||
Tests Alignment seive with CRAM input along with shifts | ||
""" | ||
output_bam_file = "/tmp/aln_seive2.bam" | ||
args = "--bam {} -o {} --minMappingQuality 10 --shift 1 -2 3 -4".format(BAMFILE_IN, output_bam_file).split() | ||
aln_seive.main(args) | ||
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bam_file_size = os.path.getsize(BAMFILE_OUT3) | ||
expected_file_size = os.path.getsize(output_bam_file) | ||
size_tolerance = 5000 | ||
size_difference = abs(bam_file_size - expected_file_size) | ||
assert size_difference <= size_tolerance, "File sizes do not match" | ||
unlink(output_bam_file) |
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please try something like this: https://github.com/deeptools/deepTools/blob/4.0.0/pydeeptools/deeptools/test/test_readFiltering.py#L76-L79