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Merge pull request #210 from nicola-debernardini/pan-Draft
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jotech authored Apr 8, 2024
2 parents 9e85ab1 + 00a1aae commit 8d4088a
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4 changes: 4 additions & 0 deletions README.md
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Expand Up @@ -82,3 +82,7 @@ GNU General Public License version 3.0 ([GPLv3](https://www.gnu.org/licenses/gpl
Licensed under the [GPL-3.0](https://www.gnu.org/licenses/gpl-3.0.en.html) license.
Link to license: https://github.com/Ecogenomics/GTDBTk/blob/master/LICENSE
Website: https://ecogenomics.github.io/GTDBTk/

## Citation
- Zimmermann, J., Kaleta, C. & Waschina, S. gapseq: informed prediction of bacterial metabolic pathways and reconstruction of accurate metabolic models. Genome Biol 22, 81 (2021). https://doi.org/10.1186/s13059-021-02295-1
- De Bernardini, N., Zampieri, G., Campanaro, S., Zimmermann, J., Waschina, S. & Treu, L. pan-Draft: Automated reconstruction of species-representative metabolic models from multiple genomes. *Currently under revision*
2 changes: 2 additions & 0 deletions docs/usage/panDraft.md
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# panDraft: species-level models

*Details on performance of pan-Draft module are currently under evaluation for pubblication*

When dealing with environmental samples, it's common for Metagenome-Assembled Genomes (MAGs) obtained from genome centric metagenomics to be incomplete and contaminated. Consequently, Genome-Scale Models (GEMs) derived from these incomplete MAGs lack a substantial portion of the metabolic potential of the corresponding species. In response, once a set of draft models is reconstructed, they can be conbined into a comprehensive model specific to the taxonomic group. This approach aims to fill the gaps present in individual models by combining homology-based searches and pan-reactome analysis.

Using `gapseq pan`, draft species-level models can be generated to enhance the representativeness of the taxa (species) metabolism.
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3 changes: 1 addition & 2 deletions src/pan-draft.R
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Expand Up @@ -38,7 +38,6 @@ for(i in parm_idx){
}
opt <- getopt(spec, args_mod)


# Help Screen
if ( !is.null(opt$help) | is.null(opt$models_path)){
cat(getopt(spec, usage=TRUE))
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mod_ids <- c(mod_ids, n_name)
model_list[[mod_idx]] <- mod
} else {
mod_ids <- c(mod_ids, mod@mod_id)
mod_ids <- c(mod_ids, mod@mod_id)
}
# Add annotation column to model attributes if not already there
mod <- add_annotation_column_to_attributes(mod)
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