Contact Information:
Zach Stevenson: zstevens at uoregon.edu
Megan Moerdyk-Schauwecker: mmoerdyk at uoregon.edu
Preprint https://www.biorxiv.org/content/10.1101/2022.10.30.514301v1
Plasmids & Sequence Files- Benchling Links:
Plasmids:
pZCS36 (https://benchling.com/s/seq-i7qw6GMJ8ThEJTEmnTJ1?m=slm-2gTnXULuDusJPi5f753D)
Heatshock Cas9 plasmid
Addgene: (link pending)
pZC41 (https://benchling.com/s/seq-tJD8NZSR0Y1BrxiJ2HwK?m=slm-5BtdVa36f40II5KQEK4E)
Heatshock Barcode Landing Pad targeting sgRNA
Addgene: (link pending)
pZC38 (https://benchling.com/s/seq-FCBHouL2KHKV3PAuzjiB?m=slm-x6vOvLImy0PCz4fHP6yZ)
G-418 Resistance Plasmid
Addgene: (link pending)
pZC32 (https://benchling.com/s/seq-XSyswxrUVO7qjVVE2P0F?m=slm-buyAkxHFJrCLOz6SFw8F)
Barcode Landing Pad Insertion Vector
Addgene: (link pending)
pZC30 (https://benchling.com/s/seq-7cdDYu8gi4IKnd8k77Sc?m=slm-z7u9zYR384ehB8Evb2Kh)
Slighly modifyed SEC plasmid for removal of let UTR
Addgene: (link pending)
pZC11 (https://benchling.com/s/seq-2rOc8VJPbNoRiKPu3dNE?m=slm-Cdso4oZ0iVaowiJhjOdY)
Empty guide RNA
Addgene: (link pending)
Strains:
PX740 (https://benchling.com/s/seq-RFFji0pMHdXo5PENvYBD?m=slm-ybkUN7bJjMTZT9b1ld1Q)
Barcode Landing Pad strain
CGC: Link Pending
PX816
TARDIS Array #1
Avalible Upon Request
PX817
TARDIS Array #2
Avalible Upon Request
PX818
TARDIS Array #3 profile 1
Avalible Upon Request
PX818
TARDIS Array #3 profile 2
Avalible Upon Request
PX786
TARDIS Array #5
Avalible Upon Request
To use these files in software like ApE, download the .gb file format
ApE can be downloaded at: https://jorgensen.biology.utah.edu/wayned/ape/
Future strains, plasmids, TARDIS libraries, etc. will be avalivble at updates to this website.
Additional supplemental files can be found on Figshare: (link here) Sequencing reads can be found at PRJNA893002 (https://www.ncbi.nlm.nih.gov/bioproject/893002)